Dimethyl sulfoxide stimulates the AhR-Jdp2 axis to control ROS accumulation in mouse embryonic fibroblasts.
AhR
Dimethyl sulfoxide
Jun dimerization protein 2
Mouse embryonic fibroblasts
Nrf2
Reactive oxygen species
Journal
Cell biology and toxicology
ISSN: 1573-6822
Titre abrégé: Cell Biol Toxicol
Pays: Switzerland
ID NLM: 8506639
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
26
10
2020
accepted:
21
02
2021
pubmed:
17
3
2021
medline:
9
4
2022
entrez:
16
3
2021
Statut:
ppublish
Résumé
The aryl hydrocarbon receptor (AhR) is a ligand-binding protein that responds to environmental aromatic hydrocarbons and stimulates the transcription of downstream phase I enzyme-related genes by binding the cis element of dioxin-responsive elements (DREs)/xenobiotic-responsive elements. Dimethyl sulfoxide (DMSO) is a well-known organic solvent that is often used to dissolve phase I reagents in toxicology and oxidative stress research experiments. In the current study, we discovered that 0.1% DMSO significantly induced the activation of the AhR promoter via DREs and produced reactive oxygen species, which induced apoptosis in mouse embryonic fibroblasts (MEFs). Moreover, Jun dimerization protein 2 (Jdp2) was found to be required for activation of the AhR promoter in response to DMSO. Coimmunoprecipitation and chromatin immunoprecipitation studies demonstrated that the phase I-dependent transcription factors, AhR and the AhR nuclear translocator, and phase II-dependent transcription factors such as nuclear factor (erythroid-derived 2)-like 2 (Nrf2) integrated into DRE sites together with Jdp2 to form an activation complex to increase AhR promoter activity in response to DMSO in MEFs. Our findings provide evidence for the functional role of Jdp2 in controlling the AhR gene via Nrf2 and provide insights into how Jdp2 contributes to the regulation of ROS production and the cell spreading and apoptosis produced by the ligand DMSO in MEFs.
Identifiants
pubmed: 33723743
doi: 10.1007/s10565-021-09592-2
pii: 10.1007/s10565-021-09592-2
pmc: PMC8986748
doi:
Substances chimiques
Ligands
0
NF-E2-Related Factor 2
0
Polychlorinated Dibenzodioxins
0
Reactive Oxygen Species
0
Receptors, Aryl Hydrocarbon
0
Dimethyl Sulfoxide
YOW8V9698H
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
203-222Informations de copyright
© 2021. The Author(s).
Références
Redox Biol. 2017 Oct;13:646-654
pubmed: 28818793
J Pharmacol Toxicol Methods. 2011 Mar-Apr;63(2):209-15
pubmed: 21059397
Environ Toxicol. 2017 Jan;32(1):311-328
pubmed: 26833863
Sci Rep. 2018 Oct 4;8(1):14828
pubmed: 30287873
Arch Biochem Biophys. 2004 Apr 15;424(2):226-34
pubmed: 15047195
J Mol Biol. 2008 Feb 29;376(4):913-25
pubmed: 18201722
Mol Cell Biol. 2005 Sep;25(18):8044-51
pubmed: 16135796
Nat Rev Mol Cell Biol. 2005 Jan;6(1):56-68
pubmed: 15688067
J Clin Aesthet Dermatol. 2012 Sep;5(9):24-6
pubmed: 23050031
Expert Opin Drug Metab Toxicol. 2019 Mar;15(3):219-229
pubmed: 30644759
Physiol Rev. 2018 Jul 1;98(3):1169-1203
pubmed: 29717933
J Cardiovasc Pharmacol. 2010 Sep;56(3):263-7
pubmed: 20571430
Chem Res Toxicol. 2008 Jul;21(7):1375-83
pubmed: 18512965
J Cell Biol. 1963 May;17:299-313
pubmed: 13985244
Toxicol Rep. 2018 Oct 04;5:1014-1020
pubmed: 30364542
Cell Death Differ. 2007 Aug;14(8):1398-405
pubmed: 17464331
Carcinogenesis. 2008 Jun;29(6):1235-43
pubmed: 18413364
J Invest Dermatol. 2012 Jan;132(1):7-9
pubmed: 22158605
Annu Rev Biomed Eng. 2005;7:105-50
pubmed: 16004568
Cell. 2006 Aug 25;126(4):663-76
pubmed: 16904174
Toxicol Appl Pharmacol. 1991 Jan;107(1):141-52
pubmed: 1987653
Methods Mol Biol. 2011;690:31-56
pubmed: 21042983
Gene. 2016 Sep 30;590(2):193-200
pubmed: 27041241
J Biol Chem. 1995 Nov 3;270(44):26292-302
pubmed: 7592839
Curr Drug Metab. 2002 Oct;3(5):481-90
pubmed: 12369894
Mol Cell Biol. 2002 Jul;22(13):4815-26
pubmed: 12052888
Mutat Res. 2004 Nov 2;555(1-2):133-48
pubmed: 15476857
Free Radic Res. 2018 May;52(5):507-543
pubmed: 29589770
Toxicol In Vitro. 2008 Dec;22(8):1832-9
pubmed: 18801422
Sci Rep. 2017 Feb 14;7:41927
pubmed: 28195146
Toxicol Sci. 2009 Oct;111(2):238-46
pubmed: 19474220
Neurourol Urodyn. 2017 Sep;36(7):1677-1684
pubmed: 28220525
Oncogene. 2010 Nov 25;29(47):6245-56
pubmed: 20802531
Adv Pharmacol. 1997;38:293-328
pubmed: 8895814
Annu Rev Pharmacol Toxicol. 1999;39:103-25
pubmed: 10331078
Curr Neurovasc Res. 2013 Nov;10(4):356-60
pubmed: 23937198
Biophys J. 2015 Jul 21;109(2):330-9
pubmed: 26200868
J Biol Chem. 2005 Aug 5;280(31):28731-41
pubmed: 15946950
Science. 2020 Jun 12;368(6496):1205-1210
pubmed: 32527825
J Biol Chem. 2005 May 27;280(21):20340-8
pubmed: 15790560
FEMS Yeast Res. 2013 Dec;13(8):820-30
pubmed: 24028688
Oncotarget. 2015 Nov 10;6(35):37737-49
pubmed: 26497998
Mol Cell Biol. 2007 Oct;27(20):7188-97
pubmed: 17709388
Can J Plast Surg. 2004 Spring;12(1):13-6
pubmed: 24115865
J Invest Dermatol. 2012 Jan;132(1):59-68
pubmed: 21753779
Skin Pharmacol Physiol. 2014;27(6):316-32
pubmed: 24994069
Mol Cell Biol. 1997 Jun;17(6):3094-102
pubmed: 9154808
Mol Biol Cell. 2009 Mar;20(6):1715-27
pubmed: 19158396
Nature. 1995 Jul 13;376(6536):167-70
pubmed: 7603567
Biochem Pharmacol. 2007 Jun 15;73(12):1853-62
pubmed: 17266942
PLoS One. 2012;7(8):e41342
pubmed: 22876286
Theranostics. 2017 Oct 17;7(19):4735-4752
pubmed: 29187900
Mol Pharmacol. 2016 Nov;90(5):674-688
pubmed: 27573671
Nat Struct Mol Biol. 2006 Apr;13(4):331-8
pubmed: 16518400
Biophys J. 2007 Sep 15;93(6):2056-68
pubmed: 17513383
Dev Biol. 2004 Jun 1;270(1):64-75
pubmed: 15136141
J Biol Chem. 1998 Feb 27;273(9):5174-83
pubmed: 9478971
Proc Natl Acad Sci U S A. 1990 Aug;87(16):6258-62
pubmed: 2166952
Arch Biochem Biophys. 2013 Sep 1;537(1):31-8
pubmed: 23800876
Cancers (Basel). 2013 Jul 26;5(3):959-84
pubmed: 24202329
FEBS Lett. 2007 Jul 31;581(19):3608-15
pubmed: 17412325
Oxid Med Cell Longev. 2018 Feb 14;2018:9524657
pubmed: 29643980
Toxicol Sci. 2012 Dec;130(2):349-61
pubmed: 22903824
Food Chem Toxicol. 1997 Dec;35(12):1135-41
pubmed: 9449218
J Cell Physiol. 1998 Mar;174(3):273-84
pubmed: 9462689
Cell Death Dis. 2013 Nov 14;4:e921
pubmed: 24232097