Rapid and efficient generation of T-cell receptor-like antibodies using chip-based single-cell analysis.
ISAAC
TCR
TCR-like antibody
tumor immunotherapy
Journal
European journal of immunology
ISSN: 1521-4141
Titre abrégé: Eur J Immunol
Pays: Germany
ID NLM: 1273201
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
02
02
2021
received:
25
11
2020
accepted:
09
03
2021
pubmed:
18
3
2021
medline:
1
10
2021
entrez:
17
3
2021
Statut:
ppublish
Résumé
Generation of TCR-like monoclonal antibodies using conventional methods is markedly laborious and inefficient. We have proposed improvements of ISAAC (chip-based Ab-secreting cell [ASC] screening method), allows comprehensive analysis of ASCs at the single-cell level to obtain TCR-like antibodies; blocking procedure enables us to avoid the detection of non-TCR-like antibodies.
Identifiants
pubmed: 33728647
doi: 10.1002/eji.202049083
doi:
Substances chimiques
Antibodies, Monoclonal
0
Receptors, Antigen, T-Cell
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1850-1853Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
Maude, S. L. et al., N. Engl. J. Med. 2014. 371: 1507-1517.
Topp, M. S. et al., J. Clin. Oncol. 2014. 32: 4134-4140.
Wittman, V. P. et al., J. Immunol. 2006. 177: 4187-4195.
Sastry, K. S. et al., J. Virol. 2011. 85: 1935-1942.
Sergeeva, A. et al., Blood 2011. 117: 4262-4272.
Dahan, R. and Reiter, Y., Expert Rev. Mol. Med. 2012. 14: e6.
Kurosawa, N. et al., Sci. Rep. 2019. 9: 9827.
Jin, A. et al., Nat. Med. 2009. 15: 1088-1092.
Ozawa, T. et al., PLoS One 2012. 7: e52383.
Hansen, T. H. J. M. C. et al., Trends Immunol. 2010. 31: 36--369.
Lyu, F. et al., N. Biotechnol. 2019. 49: 169-177.
Kondo, A. et al., J. Immunol. 1995. 155: 4307-4312.
Orcutt, K. D. et al., Protein Eng. Des. Sel. 2010. 23: 221-228.
Spiess, C. et al., Mol. Immunol. 2015. 67: 95-106.
Piao, X. et al., Oncoimmunology 2016. 5: e1131380.