Electrophysiological Phenotype Characterization of Human iPSC-Derived Neuronal Cell Lines by Means of High-Density Microelectrode Arrays.
electrophysiology
high-density microelectrode arrays
induced pluripotent stem cells
Journal
Advanced biology
ISSN: 2701-0198
Titre abrégé: Adv Biol (Weinh)
Pays: Germany
ID NLM: 101775319
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
revised:
30
11
2020
received:
24
08
2020
entrez:
17
3
2021
pubmed:
18
3
2021
medline:
26
10
2021
Statut:
ppublish
Résumé
Recent advances in the field of cellular reprogramming have opened a route to studying the fundamental mechanisms underlying common neurological disorders. High-density microelectrode-arrays (HD-MEAs) provide unprecedented means to study neuronal physiology at different scales, ranging from network through single-neuron to subcellular features. In this work, HD-MEAs are used in vitro to characterize and compare human induced-pluripotent-stem-cell-derived dopaminergic and motor neurons, including isogenic neuronal lines modeling Parkinson's disease and amyotrophic lateral sclerosis. Reproducible electrophysiological network, single-cell and subcellular metrics are used for phenotype characterization and drug testing. Metrics, such as burst shape and axonal velocity, enable the distinction of healthy and diseased neurons. The HD-MEA metrics can also be used to detect the effects of dosing the drug retigabine to human motor neurons. Finally, it is shown that the ability to detect drug effects and the observed culture-to-culture variability critically depend on the number of available recording electrodes.
Identifiants
pubmed: 33729694
doi: 10.1002/adbi.202000223
pmc: PMC7610355
mid: EMS110879
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000223Subventions
Organisme : European Research Council
ID : 694829
Pays : International
Organisme : European Research Council
ID : 875609
Pays : International
Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
Front Neurol. 2019 Feb 06;10:68
pubmed: 30787905
Cell Rep. 2014 Apr 10;7(1):1-11
pubmed: 24703839
Clin Neurophysiol. 2006 Jul;117(7):1458-66
pubmed: 16759905
Neurosci Lett. 1977 Nov;6(2-3):101-5
pubmed: 19605037
Stem Cells Transl Med. 2013 Jun;2(6):473-9
pubmed: 23658252
Adv Genet. 2015;91:1-53
pubmed: 26410029
Nat Genet. 2019 Dec;51(12):1679-1690
pubmed: 31784728
Cell Rep. 2014 Nov 20;9(4):1173-82
pubmed: 25456120
Cell Stem Cell. 2015 Mar 5;16(3):269-74
pubmed: 25732245
Proc Natl Acad Sci U S A. 2017 May 2;114(18):E3679-E3688
pubmed: 28416701
Front Neurosci. 2019 Apr 26;13:385
pubmed: 31105515
Front Neurosci. 2019 Mar 13;13:208
pubmed: 30918481
Elife. 2020 Nov 10;9:
pubmed: 33170122
Nat Commun. 2019 Nov 8;10(1):5094
pubmed: 31704946
Adv Biosyst. 2019 Feb;3(2):e1800308
pubmed: 30882024
Proc Natl Acad Sci U S A. 2019 Mar 5;116(10):4696-4705
pubmed: 30770445
J Neurosci Methods. 1980 Feb;2(1):19-31
pubmed: 7329089
Methods Mol Biol. 2016;1408:207-25
pubmed: 26965125
Sci Transl Med. 2017 May 24;9(391):
pubmed: 28539470
Neuroscience. 2014 Jul 11;272:141-53
pubmed: 24785678
Cell. 2006 Aug 25;126(4):663-76
pubmed: 16904174
IEEE J Biomed Health Inform. 2015 May;19(3):832-8
pubmed: 25069129
Hepatology. 2010 Jan;51(1):297-305
pubmed: 19998274
Stem Cells Transl Med. 2019 Dec;8(12):1272-1285
pubmed: 31631575
Integr Biol (Camb). 2015 Jan;7(1):64-72
pubmed: 25418582
Lab Chip. 2015 Jul 7;15(13):2767-80
pubmed: 25973786
Biomed Res Int. 2019 Jun 24;2019:9605265
pubmed: 31341912
Sci Rep. 2019 Nov 20;9(1):17125
pubmed: 31748598
Curr Opin Neurol. 2019 Oct;32(5):771-776
pubmed: 31361627
Elife. 2017 Oct 09;6:
pubmed: 28990925
Science. 2008 Aug 29;321(5893):1218-21
pubmed: 18669821
J Neurophysiol. 2016 Nov 1;116(5):2114-2124
pubmed: 27512022
ALTEX. 2020 May 07;37(3):350-364
pubmed: 32388569
Brain Res. 2006 Jun 6;1093(1):41-53
pubmed: 16712817
J Comput Neurosci. 2013 Jun;34(3):489-504
pubmed: 23661228
Eur J Pharmacol. 2014 Jun 5;732:68-75
pubmed: 24681057
Nat Med. 2018 Oct;24(10):1579-1589
pubmed: 30127392
J Neurosci. 2004 Feb 4;24(5):1236-44
pubmed: 14762142
Stem Cell Reports. 2015 Dec 8;5(6):1039-1052
pubmed: 26651604
Epilepsia. 2012 Mar;53(3):412-24
pubmed: 22220513
Front Neurosci. 2019 Dec 06;13:1310
pubmed: 31866818
Nat Rev Dis Primers. 2017 Mar 23;3:17013
pubmed: 28332488
J Neurophysiol. 2018 Sep 1;120(3):1212-1232
pubmed: 29847231
Cell Rep. 2016 Jun 14;15(11):2411-26
pubmed: 27264186
Nat Rev Mol Cell Biol. 2016 Mar;17(3):170-82
pubmed: 26818440
Front Comput Neurosci. 2014 Jan 13;7:193
pubmed: 24567714
CNS Drug Rev. 2005 Spring;11(1):1-20
pubmed: 15867950
J Neurosci. 2011 Jun 15;31(24):8699-705
pubmed: 21677152
Neuron. 2002 May 16;34(4):521-33
pubmed: 12062037
Lancet Neurol. 2018 Nov;17(11):928-929
pubmed: 30287052
Mol Psychiatry. 2016 Nov;21(11):1573-1588
pubmed: 27698428
Dig Tech Pap IEEE Int Solid State Circuits Conf. 2016 Feb 25;2016:394-396
pubmed: 34916732
Cell Stem Cell. 2012 Apr 6;10(4):385-97
pubmed: 22482504
Pain. 2008 Sep 15;138(3):537-545
pubmed: 18331780
Exp Cell Res. 1972 Sep;74(1):61-6
pubmed: 4672477
Cell Death Dis. 2015 Nov 26;6:e1994
pubmed: 26610207
Front Cell Neurosci. 2015 Jan 14;8:460
pubmed: 25642167
Trends Pharmacol Sci. 2020 Feb;41(2):99-109
pubmed: 31926602
IEEE J Solid-State Circuits. 2014 Nov;49(11):2705-2719
pubmed: 28502989
Front Neurosci. 2016 Mar 30;10:121
pubmed: 27065786
Sci Rep. 2019 Jun 20;9(1):8921
pubmed: 31222141
J Biomed Sci. 2019 Oct 28;26(1):87
pubmed: 31660969
Development. 2018 Mar 8;145(5):
pubmed: 29519889
Lab Chip. 2009 Feb 7;9(3):404-10
pubmed: 19156289
Stem Cell Res. 2013 Sep;11(2):743-57
pubmed: 23759711
Neurotoxicology. 2018 Jul;67:215-225
pubmed: 29909083
Neurochem Res. 2019 Jun;44(6):1475-1493
pubmed: 30989481
Sci Rep. 2019 Jul 25;9(1):10835
pubmed: 31346219
Sci Rep. 2016 May 18;6:26181
pubmed: 27188845
Methods Mol Biol. 2011;767:419-31
pubmed: 21822893
Proc Natl Acad Sci U S A. 2018 Jul 24;115(30):7813-7818
pubmed: 29991596
Cell. 2008 Sep 5;134(5):877-86
pubmed: 18691744
Nat Commun. 2013;4:2181
pubmed: 23867868
PLoS One. 2017 Oct 13;12(10):e0186147
pubmed: 29028808
Nat Commun. 2020 Sep 25;11(1):4854
pubmed: 32978383
Brain. 2006 Apr;129(Pt 4):953-62
pubmed: 16467388
Sci Rep. 2020 Mar 24;10(1):5311
pubmed: 32210279
Nat Med. 2020 Feb;26(2):289-299
pubmed: 31988461
Nat Commun. 2015 Jan 12;6:5999
pubmed: 25580746
J Biomol Screen. 2016 Jun;21(5):510-9
pubmed: 27028607
Biochem Biophys Res Commun. 2013 May 3;434(2):293-7
pubmed: 23541573
Lab Chip. 2009 Sep 21;9(18):2644-51
pubmed: 19704979
Sci Transl Med. 2012 Aug 1;4(145):145ra104
pubmed: 22855461