Fast Nanoliter-Scale Cell Assays Using Droplet Microarray-Mass Spectrometry Imaging.
MALDI imaging
MALDI mass spectrometry
droplet microarray
drug discovery
fatty acid synthase
miniaturized cell-based assay
Journal
Advanced biology
ISSN: 2701-0198
Titre abrégé: Adv Biol (Weinh)
Pays: Germany
ID NLM: 101775319
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
revised:
23
12
2020
received:
14
09
2020
entrez:
17
3
2021
pubmed:
18
3
2021
medline:
26
10
2021
Statut:
ppublish
Résumé
In pharmaceutical research and development, cell-based assays are primarily used with readout that rely on fluorescence-based and other label-dependent techniques for analysis of different cellular processes. Superhydrophobic-hydrophilic droplet microarrays (DMA) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) have recently emerged as key technologies for miniaturized high-throughput cell assays and for label-free molecular high-content drug profiling, respectively. Here, nanoliter-scale cell assays are integrated on DMAs with MALDI-MS imaging (MALDI-MSI) approaches to a droplet microarray-mass spectrometry imaging (DMA-MSI) platform. Using A549 lung cancer cells, concentration-response profiling of a pharmaceutical compound, the fatty acid synthase inhibitor GSK2194069, are demonstrated. Direct cell culture on DMAs enables combination of microscopy and high speed, high molecular content analysis using MALDI-MSI. Miniaturization of array spots down to 0.5 mm confining 40 nL droplets allows for MALDI imaging analysis of as few as ten cells per spot. Partial automation ensures a fast sample preparation workflow. Taken together, the integrated DMA-MSI platform that combines MALDI-MSI, as a label-free analytical readout, with the miniaturized droplet microarray platform is a valuable complement to high throughput cell-based assays technologies.
Identifiants
pubmed: 33729695
doi: 10.1002/adbi.202000279
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2000279Informations de copyright
© 2021 The Authors. Advanced Biology published by Wiley-VCH GmbH.
Références
W. F. An, N. Tolliday, Mol. Biotechnol. 2010, 45, 180.
a) D. C. Swinney, J. Anthony, Nat. Rev. Drug Discovery 2011, 10, 507;
b) D. T. Chiu, O. Orwar, Drug Discov. World 2004, Spring 2004, 5, 45;
c) W. E. Childers, K. M. Elokely, M. Abou-Gharbia, ACS Med. Chem. Lett. 2020, 11, 1820.
a) B. B. Aggarwal, G. Sethi, V. Baladandayuthapani, S. Krishnan, S. Shishodia, J. Cell. Biochem. 2007, 102, 580;
b) A. Luengo, D. Y. Gui, M. G. Vander Heiden, Cell Chem. Biol. 2017, 24, 1161;
c) A. Heguy, A. A. Stewart, J. D. Haley, D. E. Smith, J. G. Foulkes, Gene Expr. 1995, 4, 337;
d) L. Zhao, P. Liu, G. Boncompain, F. Loos, S. Lachkar, L. Bezu, G. Chen, H. Zhou, F. Perez, O. Kepp, G. Kroemer, Sci. Rep. 2018, 8, 14966;
e) S. Senese, Y. C. Lo, D. Huang, T. A. Zangle, A. A. Gholkar, L. Robert, B. Homet, A. Ribas, M. K. Summers, M. A. Teitell, R. Damoiseaux, J. Z. Torres, Cell Death Dis. 2014, 5, e1462.
a) M. Boutros, F. Heigwer, C. Laufer, Cell 2015, 163, 1314;
b) T. L. Riss, R. A. Moravec, A. L. Niles, S. Duellman, H. A. Benink, T. J. Worzella, et al. in Assay Guidance Manual (Eds: S. Markossian, G. S. Sittampalam, A. Grossman, K. Brimacombe, M. Arkin, D. Auld, et al.), Bethesda, MD 2004;
c) I. Becher, A. Dittmann, M. M. Savitski, C. Hopf, G. Drewes, M. Bantscheff, ACS Chem. Biol. 2014, 9, 1736.
a) I. Miller, M. Min, C. Yang, C. Tian, S. Gookin, D. Carter, S. L. Spencer, Cell Rep. 2018, 24, 1105;
b) C. Lema, A. Varela-Ramirez, R. J. Aguilera, Curr. Cell Biochem. 2011, 1, 1.
X. He, S. Memczak, J. Qu, J. C. I. Belmonte, G.-H. Liu, Nat. Metabol. 2020, 2, 293.
B. Munteanu, B. Meyer, C. von Reitzenstein, E. Burgermeister, S. Bog, A. Pahl, M. P. Ebert, C. Hopf, Anal. Chem. 2014, 86, 4642.
a) S. Schulz, M. Becker, M. R. Groseclose, S. Schadt, C. Hopf, Curr. Opin. Biotechnol. 2019, 55, 51;
b) P. Arranz-Gibert, B. Guixer, R. Prades, S. Ciudad, E. Giralt, M. Teixidó, Sci. Rep. 2019, 9, 4875;
c) D. Abu Sammour, C. Marsching, A. Geisel, K. Erich, S. Schulz, C. Ramallo Guevara, J.-H. Rabe, A. Marx, P. Findeisen, P. Hohenberger, C. Hopf, Sci. Rep. 2019, 9, 10698;
d) K. Erich, D. A. Sammour, A. Marx, C. Hopf, Biochim. Biophys. Acta, Proteins Proteomics 2017, 1865, 907;
e) I. Hinsenkamp, S. Schulz, M. Roscher, A.-M. Suhr, B. Meyer, B. Munteanu, J. Fuchser, S. O. Schoenberg, M. P. A. Ebert, B. Wängler, C. Hopf, E. Burgermeister, Neoplasia 2016, 18, 500.
D. Weigt, C. A. Parrish, J. A. Krueger, C. A. Oleykowski, A. R. Rendina, C. Hopf, Cell Chem. Biol. 2019, 26, 1322.
D. Weigt, D. A. Sammour, T. Ulrich, B. Munteanu, C. Hopf, Sci. Rep. 2018, 8, 11260.
R. E. Heap, A. Segarra-Fas, A. P. Blain, G. M. Findlay, M. Trost, Analyst 2019, 144, 6371.
M. S. Unger, L. Schumacher, T. Enzlein, D. Weigt, M. J. Zamek-Gliszczynski, M. Schwab, A. T. Nies, G. Drewes, S. Schulz, F. B. M. Reinhard, C. Hopf, Anal. Chem. 2020, 92, 11851.
K. Beeman, J. Baumgärtner, M. Laubenheimer, K. Hergesell, M. Hoffmann, U. Pehl, F. Fischer, J. C. Pieck, SLAS Discovery 2017, 22, 1203.
R. P. Simon, M. Winter, C. Kleiner, R. Ries, G. Schnapp, A. Heimann, J. Li, L. Zuvela-Jelaska, T. Bretschneider, A. H. Luippold, W. Reindl, D. Bischoff, F. H. Büttner, SLAS Discovery 2020, 25, 372.
M. Winter, R. Ries, C. Kleiner, D. Bischoff, A. H. Luippold, T. Bretschneider, F. H. Büttner, SLAS Technol. 2018, 24, 209.
a) A. J. Ibáñez, S. R. Fagerer, A. M. Schmidt, P. L. Urban, K. Jefimovs, P. Geiger, R. Dechant, M. Heinemann, R. Zenobi, Proc. Natl. Acad. Sci. U. S. A. 2013, 110, 8790;
b) J. Krismer, J. Sobek, R. F. Steinhoff, R. Brönnimann, M. Pabst, R. Zenobi, Methods Mol. Biol. 2020, 2064, 113.
K. Scupakova, F. Dewez, A. K. Walch, R. M. A. Heeren, B. Balluff, Angew. Chem. Int. Ed. 2020, 59, 17447.
a) K. D. Duncan, J. Fyrestam, I. Lanekoff, Analyst 2019, 144, 782;
b) C. L. Correa-Martínez, E. A. Idelevich, K. Sparbier, T. Kuczius, M. Kostrzewa, K. Becker, Sci. Rep. 2020, 10, 4988.
J. Koubek, O. Uhlik, K. Jecna, P. Junkova, J. Vrkoslavova, J. Lipov, V. Kurzawova, T. Macek, M. Mackova, Int. Biodeterior. Biodegrad. 2012, 69, 82.
a) K. Mallah, J. Quanico, A. Raffo-Romero, T. Cardon, S. Aboulouard, D. Devos, F. Kobeissy, K. Zibara, M. Salzet, I. Fournier, Anal. Chem. 2019, 91, 11879;
b) M. Benazouz, B. Hakim, J. L. Debrun, Rapid Commun. Mass Spectrom. 1998, 12, 1018.
a) G. P. Zeegers, B. F. Günthardt, R. Zenobi, J. Am. Soc. Mass Spectrom. 2016, 27, 699;
b) A. A. Popova, K. Demir, T. G. Hartanto, E. Schmitt, P. A. Levkin, RSC Adv. 2016, 6, 38263.
a) A. A. Popova, C. Depew, K. M. Permana, A. Trubitsyn, R. Peravali, J. Á. G. Ordiano, M. Reischl, P. A. Levkin, SLAS Technol. 2016, 22, 163;
b) A. A. Popova, S. M. Schillo, K. Demir, E. Ueda, A. Nesterov-Mueller, P. A. Levkin, Adv. Mater. 2015, 27, 5217.
A. A. Popova, K. Demir, T. G. Hartanto, E. Schmitt, P. A. Levkin, RSC Adv. 2016, 6, 38263.
a) A. A. Popova, T. Tronser, K. Demir, P. Haitz, K. Kuodyte, V. Starkuviene, P. Wajda, P. A. Levkin, Small 2019, 15, 1901299;
b) C. Haslam, J. Hellicar, A. Dunn, A. Fuetterer, N. Hardy, P. Marshall, R. Paape, M. Pemberton, A. Resemannand, M. Leveridge, J. Biomol. Screen 2016, 21, 176;
c) J. Chandler, C. Haslam, N. Hardy, M. Leveridge, P. Marshall, SLAS Discovery 2016, 22, 1262.
a) S. J. Chanock, T. Manolio, M. Boehnke, E. Boerwinkle, D. J. Hunter, G. Thomas, J. N. Hirschhorn, G. Abecasis, D. Altshuler, J. E. Bailey-Wilson, L. D. Brooks, L. R. Cardon, M. Daly, P. Donnelly, J. F. Fraumeni, Jr., N. B. Freimer, D. S. Gerhard, C. Gunter, A. E. Guttmacher, M. S. Guyer, E. L. Harris, J. Hoh, R. Hoover, C. A. Kong, K. R. Merikangas, C. C. Morton, L. J. Palmer, E. G. Phimister, J. P. Rice, J. Roberts, C. Rotimi, M. A. Tucker, K. J. Vogan, S. Wacholder, E. M. Wijsman, D. M. Winn, F. S. Collins, Nature 2007, 447, 655;
b) C. J. Lord, N. Quinn, C. J. Ryan, bioRxiv 2020, 646810.
a) S. J. Lord, K. B. Velle, R. D. Mullins, L. K. Fritz-Laylin, J. Cell Biol. 2020, 219, e202001064;
b) C. Q. Peng, Y. S. Thio, R. A. Gerhardt, Nanotechnology 2008, 19, 505603.
a) Y. Leterrier, L. Médico, F. Demarco, J. A. E. Månson, U. Betz, M. F. Escolà, M. Kharrazi Olsson, F. Atamny, Thin Solid Films 2004, 460, 156;
b) T. Agarwal, P. Biswas, S. Pal, T. K. Maiti, S. Chakraborty, S. K. Ghosh, R. Dhar, ACS Appl. Bio Mater. 2020, 3, 2522;
c) F. Tobias, J. C. McIntosh, G. J. LaBonia, M. W. Boyce, M. R. Lockett, A. B. Hummon, Anal. Chem. 2019, 91, 15370;
d) J. Li, E. H. Hill, L. Lin, Y. Zheng, ACS Nano 2019, 13, 3783;
e) M. Kopeć, S. Tas, M. Cirelli, R. van der Pol, I. de Vries, G. J. Vancso, S. de Beer, ACS Appl. Polym. Mater. 2019, 1, 136;
f) J. Rull-Barrull, M. d'Halluin, E. Le Grognec, F. X. Felpin, J. Mater. Chem. C 2017, 5, 5154.
a) F. Gentile, L. Tirinato, E. Battista, F. Causa, C. Liberale, E. M. di Fabrizio, P. Decuzzi, Biomaterials 2010, 31, 7205;
b) M. Mirbagheri, V. Adibnia, B. R. Hughes, S. D. Waldman, X. Banquy, D. K. Hwang, Mater. Horiz. 2019, 6, 45;
c) H. Shindo, M. Kuwamori, M. Taniguchi, J. Biosci. Bioeng. 2012, 113, 661.
a) M. Z. H. Khan, Cogent Eng. 2016, 3, 1170097;
b) P. K. H. Ho, M. Granström, R. H. Friend, N. C. Greenham, Adv. Mater. 1998, 10, 769.
M. Strohalm, D. Kavan, P. Novák, M. Volný, V. Havlíček, Anal. Chem. 2010, 82, 4648.