Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosis.
Amino Acid Sequence
Animals
Caenorhabditis elegans Proteins
/ chemistry
Chromosome Pairing
Crossing Over, Genetic
Fluorescent Antibody Technique
Immunohistochemistry
Male
Meiosis
/ genetics
Mutation
Nuclear Proteins
/ chemistry
Protein Binding
Protein Interaction Domains and Motifs
Synaptonemal Complex
/ metabolism
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
22
10
2020
accepted:
15
02
2021
entrez:
17
3
2021
pubmed:
18
3
2021
medline:
3
8
2021
Statut:
epublish
Résumé
During sexual reproduction the parental homologous chromosomes find each other (pair) and align along their lengths by integrating local sequence homology with large-scale contiguity, thereby allowing for precise exchange of genetic information. The Synaptonemal Complex (SC) is a conserved zipper-like structure that assembles between the homologous chromosomes, bringing them together and regulating exchanges between them. However, the molecular mechanisms by which the SC carries out these functions remain poorly understood. Here we isolated and characterized two mutations in the dimerization interface in the middle of the SC zipper in C. elegans. The mutations perturb both chromosome alignment and the regulation of genetic exchanges. Underlying the chromosome-scale phenotypes are distinct alterations to the way SC subunits interact with one another. We propose a model whereby the SC brings homologous chromosomes together through two activities: obligate zipping that prevents assembly on unpaired chromosomes; and a tendency to extend pairing interactions along the entire length of the chromosomes.
Identifiants
pubmed: 33730019
doi: 10.1371/journal.pgen.1009205
pii: PGENETICS-D-20-01621
pmc: PMC7968687
doi:
Substances chimiques
Caenorhabditis elegans Proteins
0
Nuclear Proteins
0
SYP-1 protein, C elegans
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1009205Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM128804
Pays : United States
Organisme : NICHD NIH HHS
ID : T32 HD007491
Pays : United States
Organisme : NIH HHS
ID : P40 OD010440
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA042014
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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