Structure-switching aptamer sensors for the specific detection of piperaquine and mefloquine.
Journal
Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086
Informations de publication
Date de publication:
17 03 2021
17 03 2021
Historique:
received:
03
08
2020
accepted:
22
02
2021
entrez:
18
3
2021
pubmed:
19
3
2021
medline:
13
7
2021
Statut:
ppublish
Résumé
Tracking antimalarial drug use and efficacy is essential for monitoring the current spread of antimalarial drug resistance. However, available methods for determining tablet quality and patient drug use are often inaccessible, requiring well-equipped laboratories capable of performing liquid chromatography-mass spectrometry (LC-MS). Here, we report the development of aptamer-based fluorescent sensors for the rapid, specific detection of the antimalarial compounds piperaquine and mefloquine-two slow-clearing partner drugs in current first-line artemisinin-based combination therapies (ACTs). Highly selective DNA aptamers were identified that bind piperaquine and mefloquine with dissociation constants (
Identifiants
pubmed: 33731432
pii: 13/585/eabe1535
doi: 10.1126/scitranslmed.abe1535
pii:
doi:
Substances chimiques
Antimalarials
0
Aptamers, Nucleotide
0
Quinolines
0
piperaquine
A0HV2Q956Y
Mefloquine
TML814419R
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.