Regulation of olfactomedin 4 by Porphyromonas gingivalis in a community context.
Journal
The ISME journal
ISSN: 1751-7370
Titre abrégé: ISME J
Pays: England
ID NLM: 101301086
Informations de publication
Date de publication:
09 2021
09 2021
Historique:
received:
21
10
2020
accepted:
01
03
2021
revised:
23
02
2021
pubmed:
19
3
2021
medline:
23
9
2021
entrez:
18
3
2021
Statut:
ppublish
Résumé
At mucosal barriers, the virulence of microbial communities reflects the outcome of both dysbiotic and eubiotic interactions with the host, with commensal species mitigating or potentiating the action of pathogens. We examined epithelial responses to the oral pathogen Porphyromonas gingivalis as a monoinfection and in association with a community partner, Streptococcus gordonii. RNA-Seq of oral epithelial cells showed that the Notch signaling pathway, including the downstream effector olfactomedin 4 (OLFM4), was differentially regulated by P. gingivalis alone; however, regulation was overridden by S. gordonii. OLFM4 was required for epithelial cell migratory, proliferative and inflammatory responses to P. gingivalis. Activation of Notch signaling was induced through increased expression of the Notch1 receptor and the Jagged1 (Jag1) agonist. In addition, Jag1 was released in response to P. gingivalis, leading to paracrine activation. Following Jag1-Notch1 engagement, the Notch1 extracellular domain was cleaved by P. gingivalis gingipain proteases. Antagonism by S. gordonii involved inhibition of gingipain activity by secreted hydrogen peroxide. The results establish a novel mechanism by which P. gingivalis modulates epithelial cell function which is dependent on community context. These interrelationships have relevance for innate inflammatory responses and epithelial cell fate decisions in oral health and disease.
Identifiants
pubmed: 33731837
doi: 10.1038/s41396-021-00956-4
pii: 10.1038/s41396-021-00956-4
pmc: PMC8397782
doi:
Substances chimiques
OLFM4 protein, human
0
Granulocyte Colony-Stimulating Factor
143011-72-7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
2627-2642Subventions
Organisme : NIDCR NIH HHS
ID : R01 DE011111
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE023193
Pays : United States
Organisme : NIDCR NIH HHS
ID : F30 DE028166
Pays : United States
Organisme : NIDCR NIH HHS
ID : R37 DE011111
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE028296
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE012505
Pays : United States
Organisme : NIDCR NIH HHS
ID : R00 DE028346
Pays : United States
Organisme : NIDCR NIH HHS
ID : F32 DE026939
Pays : United States
Organisme : NIDCR NIH HHS
ID : R03 DE028031
Pays : United States
Organisme : NIDCR NIH HHS
ID : K99 DE028346
Pays : United States
Organisme : NIDCR NIH HHS
ID : R01 DE017921
Pays : United States
Informations de copyright
© 2021. The Author(s), under exclusive licence to International Society for Microbial Ecology.
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