Detection and genetic characterization of African swine fever virus (ASFV) in clinically infected pigs in two districts in South Kivu province, Democratic Republic Congo.

African swine fever Democratic republic of Congo Genotype Molecular epidemiology Outbreaks Pigs

Journal

Heliyon
ISSN: 2405-8440
Titre abrégé: Heliyon
Pays: England
ID NLM: 101672560

Informations de publication

Date de publication:
Mar 2021
Historique:
received: 12 12 2020
revised: 05 01 2021
accepted: 01 03 2021
entrez: 18 3 2021
pubmed: 19 3 2021
medline: 19 3 2021
Statut: epublish

Résumé

African swine fever (ASF) is a notifiable contagious disease caused by the African swine fever virus (ASFV), leading to a serious socio-economic impact, constraining pig industry, and affecting food security worldwide. This study aimed to detect and characterize ASFV strains from suspected infected domestic pigs in two South-Kivu province districts of the Democratic Republic of the Congo (DRC). A total of 155 pig samples were screened for viral DNA and sequencing at multiple loci. An infection rate of 5.2% (8/155) was recorded from a total of 155 blood samples with the highest ASFV infection rate of 8% for Uvira (6/75) and mostly in female pigs 5 (7.6%). Most ASF associated clinical signs were redness on the skin and snout at 49% (95% CI: 21-34), followed by the unwillingness of pigs to stand at 29 % (95%, CI: 19-35). Phylogenetic analysis of partial

Identifiants

pubmed: 33732937
doi: 10.1016/j.heliyon.2021.e06419
pii: S2405-8440(21)00524-7
pmc: PMC7938247
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e06419

Informations de copyright

© 2021 The Author(s).

Références

J Gen Virol. 2018 May;99(5):613-614
pubmed: 29565243
Transbound Emerg Dis. 2017 Oct;64(5):1393-1404
pubmed: 27211823
Viruses. 2017 Feb 18;9(2):
pubmed: 28218698
Virus Res. 2013 Apr;173(1):191-7
pubmed: 23123296
J Comp Pathol. 2015 Jan;152(1):9-21
pubmed: 25443146
Vet Microbiol. 2020 Jan;240:108521
pubmed: 31902515
Acta Vet Scand. 2016 Nov 29;58(1):82
pubmed: 27899125
Virology. 1996 Jun 1;220(1):20-7
pubmed: 8659112
Virol J. 2020 Sep 3;17(1):135
pubmed: 32883295
Transbound Emerg Dis. 2018 Apr;65(2):420-431
pubmed: 28921895
Transbound Emerg Dis. 2017 Feb;64(1):101-115
pubmed: 25876769
Arch Virol. 2003 Apr;148(4):693-706
pubmed: 12664294
Vet Microbiol. 2004 Nov 15;103(3-4):169-82
pubmed: 15504588
Virus Res. 2013 Apr;173(1):212-27
pubmed: 23142551
Ecohealth. 2009 Jun;6(2):296-310
pubmed: 19915917
J Gen Virol. 2011 Feb;92(Pt 2):432-44
pubmed: 20965989
Virol J. 2013 Aug 01;10:247
pubmed: 23914918
Transbound Emerg Dis. 2011 Feb;58(1):86-92
pubmed: 21214868
Adv Virol. 2019 Apr 1;2019:1463245
pubmed: 31057618
Mol Biol Evol. 2016 Jul;33(7):1870-4
pubmed: 27004904
BMC Vet Res. 2013 Dec 26;9:263
pubmed: 24369729
Viruses. 2017 May 10;9(5):
pubmed: 28489063
Arch Virol. 2006 Dec;151(12):2475-94
pubmed: 16817033
Virus Genes. 2009 Feb;38(1):85-95
pubmed: 19009341

Auteurs

Patrick N Bisimwa (PN)

Université Evangélique en Afrique, Department of Animal Science and Production, Biosciences Unit, Bukavu, Democratic Republic of the Congo.
Pan African University Institute of Basic Sciences, Technology and Innovation, Department of Molecular Biology and Biotechnology, Nairobi, Kenya.

Lionel K Ishara (LK)

Université Evangélique en Afrique, Department of Animal Science and Production, Biosciences Unit, Bukavu, Democratic Republic of the Congo.

Dieudonné S Wasso (DS)

Université Evangélique en Afrique, Department of Animal Science and Production, Biosciences Unit, Bukavu, Democratic Republic of the Congo.

Fabrice Bantuzeko (F)

Université Evangélique en Afrique, Department of Animal Science and Production, Biosciences Unit, Bukavu, Democratic Republic of the Congo.

Ronald Tonui (R)

Pan African University Institute of Basic Sciences, Technology and Innovation, Department of Molecular Biology and Biotechnology, Nairobi, Kenya.

Ahadi B Bwihangane (AB)

Université Evangélique en Afrique, Department of Animal Science and Production, Biosciences Unit, Bukavu, Democratic Republic of the Congo.

Classifications MeSH