Multilayer and MATR3-dependent regulation of mRNAs maintains pluripotency in human induced pluripotent stem cells.

cellular neuroscience molecular biology stem cells research

Journal

iScience
ISSN: 2589-0042
Titre abrégé: iScience
Pays: United States
ID NLM: 101724038

Informations de publication

Date de publication:
19 Mar 2021
Historique:
received: 18 05 2020
revised: 22 11 2020
accepted: 11 02 2021
entrez: 18 3 2021
pubmed: 19 3 2021
medline: 19 3 2021
Statut: epublish

Résumé

Matrin3 (MATR3) is a nuclear RNA/DNA-binding protein that plays pleiotropic roles in gene expression regulation by directly stabilizing target RNAs and supporting the activity of transcription factors by modulating chromatin architecture. MATR3 is involved in the differentiation of neural cells, and, here, we elucidate its critical functions in regulating pluripotent circuits in human induced pluripotent stem cells (hiPSCs). MATR3 downregulation affects hiPSCs' differentiation potential by altering key pluripotency regulators' expression levels, including OCT4, NANOG, and LIN28A by pleiotropic mechanisms. MATR3 binds to the

Identifiants

pubmed: 33733063
doi: 10.1016/j.isci.2021.102197
pii: S2589-0042(21)00165-6
pmc: PMC7940987
doi:

Types de publication

Journal Article

Langues

eng

Pagination

102197

Informations de copyright

© 2021 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interest.

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Auteurs

Daniele Pollini (D)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Rosa Loffredo (R)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Federica Maniscalco (F)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.
Institute of Biophysics, CNR, Trento, Italy.

Marina Cardano (M)

Cell Technology Core Facility, Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Mariachiara Micaelli (M)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Isabelle Bonomo (I)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Nausicaa Valentina Licata (NV)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Daniele Peroni (D)

Mass Spectrometry Core Facility, Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Weronika Tomaszewska (W)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Annalisa Rossi (A)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Valeria Crippa (V)

Laboratorio di Biologia Applicata, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.

Erik Dassi (E)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Gabriella Viero (G)

Institute of Biophysics, CNR, Trento, Italy.

Alessandro Quattrone (A)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Angelo Poletti (A)

Laboratorio di Biologia Applicata, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, Milan, Italy.

Luciano Conti (L)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Alessandro Provenzani (A)

Department of Cellular, Computational and Integrative Biology, University of Trento, Trento, Italy.

Classifications MeSH