Detection of pathological alpha-synuclein aggregates in human iPSC-derived neurons and tissue.
Molecular biology
Neuroscience
Protein biochemistry
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
19 03 2021
19 03 2021
Historique:
entrez:
18
3
2021
pubmed:
19
3
2021
medline:
19
3
2021
Statut:
epublish
Résumé
The accumulation of proteins into insoluble aggregates is a common feature of several neurodegenerative diseases. Aggregated α-synuclein is a major component of Lewy bodies that pathologically define Parkinson's disease (PD). Here, we present methods for the detection of pathogenic conformations of α-synuclein in induced pluripotent stem cell (iPSC) patient-derived neuron models and brain tissue. These methods can be applied to studies of PD pathogenesis and the discovery of novel therapeutics that restore physiological α-synuclein. For complete details on the use and execution of this protocol, please refer to Cuddy et al. (2019) and Zunke et al. (2018).
Identifiants
pubmed: 33733241
doi: 10.1016/j.xpro.2021.100372
pii: S2666-1667(21)00079-4
pmc: PMC7941090
doi:
Substances chimiques
alpha-Synuclein
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Pagination
100372Subventions
Organisme : NINDS NIH HHS
ID : R01 NS092823
Pays : United States
Organisme : NINDS NIH HHS
ID : RF1 NS109157
Pays : United States
Informations de copyright
© 2021 The Author(s).
Déclaration de conflit d'intérêts
J.R.M. has received consulting fees from SK Biopharmaceuticals, Neuron23, Sanofi-Genzyme, and Lysosomal Therapeutics. J.R.M. has ownership/investment interests in Lysosomal Therapeutics.
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