Immunity improvement and gut microbiota remodeling of mice by wheat germ globulin.
Gut microbiota
Immunity improvement
Mice
Wheat germ globulin
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
18 Mar 2021
18 Mar 2021
Historique:
received:
12
01
2021
accepted:
08
03
2021
entrez:
18
3
2021
pubmed:
19
3
2021
medline:
13
7
2021
Statut:
epublish
Résumé
The wheat germ protein (WG) and it's proteolytic peptide have a variety of biological activities. Our previous work showed that WG could improve immunity of the immunosuppressive mice established by cyclophosphamide. However, in the healthy condition and normal diet, as a supplementary food, the effects of immunity improvement and gut microbiota remodeling by the wheat germ globulin has not been studied yet. Here, we reported that WG could improve the immunity and remodel the gut microbiota of the mice, as a potentially safe functional supplementary food for the first time. The increase of interleukin-6 (IL-6) and the decrease of tumor necrosis factor α (TNF-α) and interleukin-10 (IL-10) indicated that WG could enhance the levels of activated T cells and monocytes and anti-inflammatory ability, meanwhile, the significant increase of immunoglobin G (lgG) and the notable decrease of the immunoglobin M (lgM) and immunoglobin A (lgA) illustrated that WG could improve immunity by promoting the differentiation and maturation process of B cells, compared with the NC group (normal control group). 16S rRNA sequencing showed WG could remodel the gut microbiota. At the phylum level, the Bacteroidetes were reduced and Firmicutes were increased in WG group, compared with NC group. At the genus level, the SCFA producing genera of unclassified_f_Lachnospiraceae, Blautia and especially the Roseburia (increased more than threefold) increased notably. Further, the level changes of cytokines and immunoglobulins were associated with the gut microbiota. This work showed that WG could improve immunity and has potential application value as an immune-enhancing functional food.
Identifiants
pubmed: 33733383
doi: 10.1007/s11274-021-03034-1
pii: 10.1007/s11274-021-03034-1
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Cytokines
0
Immunoglobulin G
0
Immunoglobulins
0
RNA, Ribosomal, 16S
0
Tumor Necrosis Factor-alpha
0
Interleukin-10
130068-27-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
64Subventions
Organisme : Zhongyuan Scholars in Henan
ID : No. 192101510004
Organisme : Major Public Welfare Projects of Henan Province
ID : 201300110300
Organisme : Consulting Research Project of Henan Institute of Chinese Engineering Development Strategies
ID : NO. 2020HENZT13
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