Potential of Magnetic Hyperthermia to Stimulate Localized Immune Activation.

biological response heat-shock protein 70 immune stimulation magnetic hyperthermia superparamagnetic iron oxide nanoparticles

Journal

Small (Weinheim an der Bergstrasse, Germany)
ISSN: 1613-6829
Titre abrégé: Small
Pays: Germany
ID NLM: 101235338

Informations de publication

Date de publication:
04 2021
Historique:
revised: 20 01 2021
received: 25 08 2020
pubmed: 19 3 2021
medline: 15 7 2021
entrez: 18 3 2021
Statut: ppublish

Résumé

Magnetic hyperthermia (MH) harnesses the heat-releasing properties of superparamagnetic iron oxide nanoparticles (SPIONs) and has potential to stimulate immune activation in the tumor microenvironment whilst sparing surrounding normal tissues. To assess feasibility of localized MH in vivo, SPIONs are injected intratumorally and their fate tracked by Zirconium-89-positron emission tomography, histological analysis, and electron microscopy. Experiments show that an average of 49% (21-87%, n = 9) of SPIONs are retained within the tumor or immediately surrounding tissue. In situ heating is subsequently generated by exposure to an externally applied alternating magnetic field and monitored by thermal imaging. Tissue response to hyperthermia, measured by immunohistochemical image analysis, reveals specific and localized heat-shock protein expression following treatment. Tumor growth inhibition is also observed. To evaluate the potential effects of MH on the immune landscape, flow cytometry is used to characterize immune cells from excised tumors and draining lymph nodes. Results show an influx of activated cytotoxic T cells, alongside an increase in proliferating regulatory T cells, following treatment. Complementary changes are found in draining lymph nodes. In conclusion, results indicate that biologically reactive MH is achievable in vivo and can generate localized changes consistent with an anti-tumor immune response.

Identifiants

pubmed: 33734595
doi: 10.1002/smll.202005241
doi:

Substances chimiques

Ferric Compounds 0
Magnetite Nanoparticles 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e2005241

Subventions

Organisme : Medical Research Council
ID : G1001497
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT 088641/Z/09/Z
Pays : United Kingdom
Organisme : Cancer Research UK Accelerator
ID : Cl 15121 A 20256

Informations de copyright

© 2021 The Authors. Small published by Wiley-VCH GmbH.

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Auteurs

Thomas J Carter (TJ)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.

Giulia Agliardi (G)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.

Fang-Yu Lin (FY)

UCL Healthcare Biomagnetics Laboratory, 21 Albermarle Street, London, W1S 4BS, UK.

Matthew Ellis (M)

Division of Neuropathology, Department of Neurodegenerative Disease, UCL Queen Square Institute of Neurology, Queen Square, London, WC1N 3BG, UK.
Cancer Sciences Unit, Cancer Research UK Centre, University of Southampton, Somers Building, Southampton, SO16 6YD, UK.

Clare Jones (C)

School of Biomedical Engineering and Imaging Sciences, King's College London (KCL), St Thomas' Hospital, London, SE1 7EH, UK.

Mathew Robson (M)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.

Angela Richard-Londt (A)

Division of Neuropathology, Department of Neurodegenerative Disease, UCL Queen Square Institute of Neurology, Queen Square, London, WC1N 3BG, UK.

Paul Southern (P)

UCL Healthcare Biomagnetics Laboratory, 21 Albermarle Street, London, W1S 4BS, UK.
Resonant Circuits Limited (RCL), London, W1S 4BS, UK.

Mark Lythgoe (M)

Centre for Advanced Biomedical Imaging, Division of Medicine, University College London, London, WC1E 6DD, UK.

May Zaw Thin (M)

Centre for Advanced Biomedical Imaging, Division of Medicine, University College London, London, WC1E 6DD, UK.

Vyacheslav Ryzhov (V)

NRC "Kurchatov Institute", Petersburg Nuclear Physics Institute, Gatchina, 188300, Russia.

Rafael T M de Rosales (RTM)

School of Biomedical Engineering and Imaging Sciences, King's College London (KCL), St Thomas' Hospital, London, SE1 7EH, UK.

Cordula Gruettner (C)

Micromod Partikeltechnologie GmbH, Friedrich-Barnewitz-Str. 4, Rostock, D-18119, Germany.

Maha R A Abdollah (MRA)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.
Department of Pharmacology and Biochemistry, Faculty of Pharmacy, The British University in Egypt (BUE), El Shorouk City, Misr- Ismalia Desert Road, 11873, Cairo, Egypt.

R Barbara Pedley (RB)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.

Quentin A Pankhurst (QA)

UCL Healthcare Biomagnetics Laboratory, 21 Albermarle Street, London, W1S 4BS, UK.
Resonant Circuits Limited (RCL), London, W1S 4BS, UK.

Tammy L Kalber (TL)

Centre for Advanced Biomedical Imaging, Division of Medicine, University College London, London, WC1E 6DD, UK.

Sebastian Brandner (S)

Division of Neuropathology, Department of Neurodegenerative Disease, UCL Queen Square Institute of Neurology, Queen Square, London, WC1N 3BG, UK.

Sergio Quezada (S)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.

Paul Mulholland (P)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.

Maxim Shevtsov (M)

NRC "Kurchatov Institute", Petersburg Nuclear Physics Institute, Gatchina, 188300, Russia.
Technical University of Munich, Klinikum Rechts der Isar, Ismaninger str. 22, Munich, 81675, Germany.

Kerry Chester (K)

UCL Cancer Institute, University College London (UCL), Paul O'Gorman Building, 72 Huntley Street, London, WC1E 6DD, UK.

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