Oxygen enhancement ratios of cancer cells after exposure to intensity modulated x-ray fields: DNA damage and cell survival.
DNA damage
cell survival
intensity modulated radiation fields
oxygen enhancement ratio
Journal
Physics in medicine and biology
ISSN: 1361-6560
Titre abrégé: Phys Med Biol
Pays: England
ID NLM: 0401220
Informations de publication
Date de publication:
06 04 2021
06 04 2021
Historique:
received:
18
12
2020
accepted:
18
03
2021
pubmed:
19
3
2021
medline:
9
10
2021
entrez:
18
3
2021
Statut:
epublish
Résumé
Hypoxic cancer cells within solid tumours show radio-resistance, leading to malignant progression in fractionated radiotherapy. When prescribing dose to tumours under heterogeneous oxygen pressure with intensity-modulated radiation fields, intercellular signalling could have an impact on radiosensitivity between in-field and out-of-field (OF) cells. However, the impact of hypoxia on radio-sensitivity under modulated radiation intensity remains to be fully clarified. Here, we investigate the impact of hypoxia on in-field and OF radio-sensitivities using two types of cancer cells, DU145 and H1299. Using a nBIONIX hypoxic culture kit and a shielding technique to irradiate 50% of a cell culture flask, oxygen enhancement ratios for double-strand breaks (DSB) and cell death endpoints were determined. These
Identifiants
pubmed: 33735839
doi: 10.1088/1361-6560/abf011
doi:
Substances chimiques
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2021 Institute of Physics and Engineering in Medicine.