Differentiation of Hodgkin lymphoma cells by reactive oxygen species and regulation by heme oxygenase-1 through HIF-1α.


Journal

Cancer science
ISSN: 1349-7006
Titre abrégé: Cancer Sci
Pays: England
ID NLM: 101168776

Informations de publication

Date de publication:
Jun 2021
Historique:
revised: 07 03 2021
received: 09 11 2020
accepted: 15 03 2021
pubmed: 20 3 2021
medline: 11 6 2021
entrez: 19 3 2021
Statut: ppublish

Résumé

We previously indicated that Hodgkin lymphoma (HL) cells contain a small side population (SP) that differentiate into a large major population (MP) with giant Hodgkin and Reed-Sternberg (H and RS)-like cells. However, its molecular mechanisms are not fully understood. In this study, we found that intracellular reactive oxygen species (ROS) are low in the SP compared to the MP. Hydrogen peroxide induces large H- and RS-like cells in HL cell lines, but induces cell death in unrelated lymphoid cell lines. Microarray analyses revealed the enrichment of upregulated genes under hypoxic conditions in the SP compared to the MP, and we verified that the SP cells are hypoxic. Hypoxia inducible factor (HIF)-1α was preferentially expressed in the SP. CoCl

Identifiants

pubmed: 33738869
doi: 10.1111/cas.14890
pmc: PMC8177765
doi:

Substances chimiques

HIF1A protein, human 0
Hypoxia-Inducible Factor 1, alpha Subunit 0
Protoporphyrins 0
Reactive Oxygen Species 0
zinc protoporphyrin 15442-64-5
Cobalt 3G0H8C9362
Hydrogen Peroxide BBX060AN9V
HMOX1 protein, human EC 1.14.14.18
Heme Oxygenase-1 EC 1.14.14.18
cobaltous chloride EVS87XF13W

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

2542-2555

Subventions

Organisme : Japan Agency for Medical Research and Development
ID : 19fk0108039h0003
Organisme : Japan Agency for Medical Research and Development
ID : 20fk0108126h0001
Organisme : Japan Society for the Promotion of Science
ID : 17K08728
Organisme : Japan Society for the Promotion of Science
ID : 19K07442
Organisme : Japan Society for the Promotion of Science
ID : 19K16580
Organisme : Japan Society for the Promotion of Science
ID : 20K07379

Informations de copyright

© 2021 The Authors. Cancer Science published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.

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Auteurs

Makoto Nakashima (M)

Laboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.

Mariko Watanabe (M)

Divison of Hematology, Department of Laboratory Sciences, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.

Kazumi Nakano (K)

Laboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.

Kaoru Uchimaru (K)

Laboratory of Tumor Cell Biology, Department of Computational Biology and Medical Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo, Japan.

Ryouichi Horie (R)

Divison of Hematology, Department of Laboratory Sciences, School of Allied Health Sciences, Kitasato University, Sagamihara, Japan.

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Classifications MeSH