Inside-out chicken enteroids with leukocyte component as a model to study host-pathogen interactions.
Animals
Cells, Cultured
Cellular Microenvironment
Chickens
Eimeria tenella
/ immunology
Epithelial Cells
/ immunology
Host-Pathogen Interactions
Influenza A virus
/ immunology
Intestinal Mucosa
/ immunology
Leukocytes
/ immunology
Mice, Inbred C57BL
Organoids
Permeability
Phagocytosis
Phenotype
Quail
Salmonella typhimurium
/ immunology
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
19 03 2021
19 03 2021
Historique:
received:
21
05
2020
accepted:
23
02
2021
entrez:
20
3
2021
pubmed:
21
3
2021
medline:
12
8
2021
Statut:
epublish
Résumé
Mammalian three-dimensional (3D) enteroids mirror in vivo intestinal organisation and are powerful tools to investigate intestinal cell biology and host-pathogen interactions. We have developed complex multilobulated 3D chicken enteroids from intestinal embryonic villi and adult crypts. These avian enteroids develop optimally in suspension without the structural support required to produce mammalian enteroids, resulting in an inside-out enteroid conformation with media-facing apical brush borders. Histological and transcriptional analyses show these enteroids comprise of differentiated intestinal epithelial cells bound by cell-cell junctions, and notably, include intraepithelial leukocytes and an inner core of lamina propria leukocytes. The advantageous polarisation of these enteroids has enabled infection of the epithelial apical surface with Salmonella Typhimurium, influenza A virus and Eimeria tenella without the need for micro-injection. We have created a comprehensive model of the chicken intestine which has the potential to explore epithelial and leukocyte interactions and responses in host-pathogen, food science and pharmaceutical research.
Identifiants
pubmed: 33742093
doi: 10.1038/s42003-021-01901-z
pii: 10.1038/s42003-021-01901-z
pmc: PMC7979936
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
377Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/D/20320000
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/D/10002071
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BBS/E/D/20002173
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/MO14819
Pays : United Kingdom
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