Insights into the genome and secretome of Fusarium metavorans DSM105788 by cultivation on agro-residual biomass and synthetic nutrient sources.
CAZyme analysis
Cellulose degradation
Fusarium metavorans
Fusarium solani species complex
Genome analysis
Mass spectrometry
Proteomics
Residual biomass treatment
Secretome
Journal
Biotechnology for biofuels
ISSN: 1754-6834
Titre abrégé: Biotechnol Biofuels
Pays: England
ID NLM: 101316935
Informations de publication
Date de publication:
20 Mar 2021
20 Mar 2021
Historique:
received:
17
11
2020
accepted:
11
03
2021
entrez:
21
3
2021
pubmed:
22
3
2021
medline:
22
3
2021
Statut:
epublish
Résumé
The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to monosaccharides. In nature, lignocellulosic biomass is mainly decomposed by fungi. We recently identified six efficient cellulose degraders by screening fungi from Vietnam. We characterized a high-performance cellulase-producing strain, with an activity of 0.06 U/mg, which was identified as a member of the Fusarium solani species complex linkage 6 (Fusarium metavorans), isolated from mangrove wood (FW16.1, deposited as DSM105788). The genome, representing nine potential chromosomes, was sequenced using PacBio and Illumina technology. In-depth secretome analysis using six different synthetic and artificial cellulose substrates and two agro-industrial waste products identified 500 proteins, including 135 enzymes assigned to five different carbohydrate-active enzyme (CAZyme) classes. The F. metavorans enzyme cocktail was tested for saccharification activity on pre-treated sugarcane bagasse, as well as untreated sugarcane bagasse and maize leaves, where it was complemented with the commercial enzyme mixture Accellerase 1500. In the untreated sugarcane bagasse and maize leaves, initial cell wall degradation was observed in the presence of at least 196 µg/mL of the in-house cocktail. Increasing the dose to 336 µg/mL facilitated the saccharification of untreated sugarcane biomass, but had no further effect on the pre-treated biomass. Our results show that F. metavorans DSM105788 is a promising alternative pre-treatment for the degradation of agro-industrial lignocellulosic materials. The enzyme cocktail promotes the debranching of biopolymers surrounding the cellulose fibers and releases reduced sugars without process disadvantages or loss of carbohydrates.
Sections du résumé
BACKGROUND
BACKGROUND
The transition to a biobased economy involving the depolymerization and fermentation of renewable agro-industrial sources is a challenge that can only be met by achieving the efficient hydrolysis of biomass to monosaccharides. In nature, lignocellulosic biomass is mainly decomposed by fungi. We recently identified six efficient cellulose degraders by screening fungi from Vietnam.
RESULTS
RESULTS
We characterized a high-performance cellulase-producing strain, with an activity of 0.06 U/mg, which was identified as a member of the Fusarium solani species complex linkage 6 (Fusarium metavorans), isolated from mangrove wood (FW16.1, deposited as DSM105788). The genome, representing nine potential chromosomes, was sequenced using PacBio and Illumina technology. In-depth secretome analysis using six different synthetic and artificial cellulose substrates and two agro-industrial waste products identified 500 proteins, including 135 enzymes assigned to five different carbohydrate-active enzyme (CAZyme) classes. The F. metavorans enzyme cocktail was tested for saccharification activity on pre-treated sugarcane bagasse, as well as untreated sugarcane bagasse and maize leaves, where it was complemented with the commercial enzyme mixture Accellerase 1500. In the untreated sugarcane bagasse and maize leaves, initial cell wall degradation was observed in the presence of at least 196 µg/mL of the in-house cocktail. Increasing the dose to 336 µg/mL facilitated the saccharification of untreated sugarcane biomass, but had no further effect on the pre-treated biomass.
CONCLUSION
CONCLUSIONS
Our results show that F. metavorans DSM105788 is a promising alternative pre-treatment for the degradation of agro-industrial lignocellulosic materials. The enzyme cocktail promotes the debranching of biopolymers surrounding the cellulose fibers and releases reduced sugars without process disadvantages or loss of carbohydrates.
Identifiants
pubmed: 33743779
doi: 10.1186/s13068-021-01927-9
pii: 10.1186/s13068-021-01927-9
pmc: PMC7981871
doi:
Types de publication
Journal Article
Langues
eng
Pagination
74Subventions
Organisme : Bundesministerium für Bildung und Forschung
ID : 03A0150B
Références
FEBS Lett. 2009 Apr 17;583(8):1323-6
pubmed: 19306878
Adv Protein Chem Struct Biol. 2015;98:223-61
pubmed: 25819281
Curr Protoc Mol Biol. 2001 May;Chapter 2:Unit2.3
pubmed: 18265183
Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):13822-7
pubmed: 25201969
Plant Physiol. 2010 Jun;153(2):444-55
pubmed: 20406913
Biochem J. 2004 Sep 15;382(Pt 3):769-81
pubmed: 15214846
Bioresour Technol. 2016 Jan;199:49-58
pubmed: 26321216
Nat Protoc. 2006;1(6):2856-60
pubmed: 17406544
BMC Bioinformatics. 2011 May 15;12:159
pubmed: 21575167
Biotechnol Biofuels. 2012 Jul 26;5(1):52
pubmed: 22835028
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
PLoS One. 2012;7(4):e32990
pubmed: 22496740
Crit Rev Biotechnol. 2002;22(4):375-407
pubmed: 12487426
Carbohydr Res. 1990 Apr 25;200:9-31
pubmed: 2379217
Biotechnol Bioeng. 2011 Oct;108(10):2261-9
pubmed: 21455933
AMB Express. 2016 Dec;6(1):103
pubmed: 27807811
Folia Microbiol (Praha). 2012 May;57(3):221-7
pubmed: 22488104
Sci Rep. 2016 Nov 21;6:37356
pubmed: 27869125
Biotechnol Biofuels. 2019 Feb 27;12:42
pubmed: 30858879
Appl Environ Microbiol. 2013 Jan;79(2):488-96
pubmed: 23124232
Mycologia. 2011 Nov-Dec;103(6):1161-74
pubmed: 21700639
FEBS J. 2016 May;283(9):1701-19
pubmed: 26929175
PLoS One. 2012;7(6):e28742
pubmed: 22719820
Biotechnol Biofuels. 2013 Nov 29;6(1):172
pubmed: 24286470
Emerg Microbes Infect. 2016 Dec 7;5(12):e124
pubmed: 27924809
PLoS One. 2018 Aug 30;13(8):e0202695
pubmed: 30161149
Nat Rev Genet. 2008 Jun;9(6):433-43
pubmed: 18487988
Annu Rev Microbiol. 2013;67:477-98
pubmed: 23808333
J Appl Microbiol. 2018 Sep;125(3):632-645
pubmed: 29786939
Clin Microbiol Rev. 1994 Oct;7(4):479-504
pubmed: 7834602
BMC Genomics. 2012 Jul 19;13:321
pubmed: 22812459
Chem Soc Rev. 2011 Nov;40(11):5266-81
pubmed: 21713268
Nat Commun. 2015 Jan 22;6:5961
pubmed: 25608804
Biodegradation. 2001;12(4):235-45
pubmed: 11826906
Appl Microbiol Biotechnol. 2011 Sep;91(6):1477-92
pubmed: 21785931
mSphere. 2020 Sep 16;5(5):
pubmed: 32938701
Enzyme Res. 2011;2011:217861
pubmed: 21755038
ACS Chem Biol. 2011 Dec 16;6(12):1399-406
pubmed: 22004347
PLoS One. 2015 Jun 08;10(6):e0129275
pubmed: 26053961
Bioresour Technol. 2013 Jan;127:500-7
pubmed: 23069613
Bioresour Technol. 2016 Jan;199:76-82
pubmed: 26320388
Cold Spring Harb Protoc. 2010 Sep 01;2010(9):pdb.ip80
pubmed: 20810624
Stand Genomic Sci. 2015 Jul 30;10:46
pubmed: 26380634
Nucleic Acids Res. 2014 Jan;42(Database issue):D222-30
pubmed: 24288371
Int J Mol Cell Med. 2013 Spring;2(2):64-71
pubmed: 24551793
Science. 2006 Jan 27;311(5760):484-9
pubmed: 16439654
Curr Opin Plant Biol. 2008 Jun;11(3):266-77
pubmed: 18486536
Biotechnol Biofuels. 2016 Mar 17;9:66
pubmed: 26989443
Curr Opin Biotechnol. 2014 Jun;27:150-8
pubmed: 24549148
Genome Announc. 2017 Oct 19;5(42):
pubmed: 29051243
J Bacteriol. 2014 Dec;196(23):4103-10
pubmed: 25225266
Fungal Genet Biol. 2013 Mar;52:20-31
pubmed: 23357352
J Bacteriol. 1960 Jun;79:816-26
pubmed: 14420566
Medicine (Baltimore). 2013 Nov;92(6):305-316
pubmed: 24145697
Bioresour Technol. 2013 Mar;131:500-7
pubmed: 23391738
PLoS Comput Biol. 2016 Jun 16;12(6):e1004753
pubmed: 27308864
FEBS J. 2005 Nov;272(22):5923-39
pubmed: 16279955
Nucleic Acids Res. 2012 Jul;40(Web Server issue):W445-51
pubmed: 22645317
Am J Bot. 2011 Mar;98(3):426-38
pubmed: 21613136
Bioinformatics. 2012 Jan 1;28(1):125-6
pubmed: 22039206
Proc Natl Acad Sci U S A. 2018 Oct 16;115(42):10642-10647
pubmed: 30275304
PLoS One. 2014 Aug 18;9(8):e103580
pubmed: 25133818
BMC Genomics. 2013 Apr 23;14:274
pubmed: 23617724
Mol Plant Pathol. 2016 Feb;17(2):146-58
pubmed: 26531837
PLoS Genet. 2009 Aug;5(8):e1000618
pubmed: 19714214
Bioresour Technol. 2001 May;77(3):215-27
pubmed: 11272008
Science. 2007 Feb 9;315(5813):804-7
pubmed: 17289988
Annu Rev Chem Biomol Eng. 2011;2:121-45
pubmed: 22432613
Biotechnol Prog. 2016 Mar;32(2):327-36
pubmed: 26697775
Nucleic Acids Res. 2018 Jul 2;46(W1):W95-W101
pubmed: 29771380
Nucleic Acids Res. 2014 Jan;42(Database issue):D490-5
pubmed: 24270786