Bioinspired artificial exosomes based on lipid nanoparticles carrying let-7b-5p promote angiogenesis in vitro and in vivo.
angiogenesis
endothelial cells
extracellular vesicles
let-7b-5p
microRNAs
nanomedicine
Journal
Molecular therapy : the journal of the American Society of Gene Therapy
ISSN: 1525-0024
Titre abrégé: Mol Ther
Pays: United States
ID NLM: 100890581
Informations de publication
Date de publication:
07 07 2021
07 07 2021
Historique:
received:
12
03
2020
revised:
18
01
2021
accepted:
15
03
2021
pubmed:
22
3
2021
medline:
18
1
2022
entrez:
21
3
2021
Statut:
ppublish
Résumé
MicroRNAs (miRNAs) regulate gene expression by post-transcriptional inhibition of target genes. Proangiogenic small extracellular vesicles (sEVs; popularly identified with the name "exosomes") with a composite cargo of miRNAs are secreted by cultured stem cells and present in human biological fluids. Lipid nanoparticles (LNPs) represent an advanced platform for clinically approved delivery of RNA therapeutics. In this study, we aimed to (1) identify the miRNAs responsible for sEV-induced angiogenesis; (2) develop the prototype of bioinspired "artificial exosomes" (AEs) combining LNPs with a proangiogenic miRNA, and (3) validate the angiogenic potential of the bioinspired AEs. We previously reported that human sEVs from bone marrow (BM)-CD34
Identifiants
pubmed: 33744469
pii: S1525-0016(21)00144-1
doi: 10.1016/j.ymthe.2021.03.015
pmc: PMC8261169
pii:
doi:
Substances chimiques
Lipid Nanoparticles
0
Liposomes
0
MicroRNAs
0
mirnlet7 microRNA, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2239-2252Subventions
Organisme : British Heart Foundation
ID : RG/20/9/35101
Pays : United Kingdom
Organisme : British Heart Foundation
ID : PG/11/67/29067
Pays : United Kingdom
Organisme : British Heart Foundation
ID : PG/18/31/33759
Pays : United Kingdom
Organisme : Department of Health
Pays : United Kingdom
Organisme : British Heart Foundation
ID : CH/15/1/31199
Pays : United Kingdom
Organisme : British Heart Foundation
ID : CH/1992027/7163
Pays : United Kingdom
Informations de copyright
Copyright © 2021 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
Références
J Proteome Res. 2008 Dec;7(12):5157-66
pubmed: 19367702
Mol Cell Biol. 2017 Jul 28;37(16):
pubmed: 28584193
JCI Insight. 2019 Nov 1;4(21):
pubmed: 31581150
Circ Res. 2011 Sep 16;109(7):724-8
pubmed: 21835908
Biochim Biophys Acta. 2013 Jul;1831(7):1302-9
pubmed: 24046871
Basic Res Cardiol. 1998;93 Suppl 3:8-12
pubmed: 9879436
Stem Cells Transl Med. 2015 May;4(5):513-22
pubmed: 25824139
Vascul Pharmacol. 2015 Aug;71:24-30
pubmed: 25869502
J Extracell Vesicles. 2019 Aug 1;8(1):1648167
pubmed: 31489144
Nucleic Acids Res. 2012 Oct;40(18):8818-34
pubmed: 22798498
Nucleic Acids Res. 2014 Jul;42(12):7539-52
pubmed: 24875477
Circulation. 2010 Apr 20;121(15):1735-45
pubmed: 20368523
Mol Cancer. 2013 Jun 14;12:62
pubmed: 23767874
Stem Cells Int. 2018 Sep 9;2018:3290372
pubmed: 30271437
J Mol Cell Cardiol. 1996 Sep;28(9):2005-16
pubmed: 8899559
Stem Cell Res. 2010 May;4(3):214-22
pubmed: 20138817
ACS Nano. 2015 Feb 24;9(2):1581-91
pubmed: 25558928
Stem Cells. 2017 Apr;35(4):851-858
pubmed: 28294454
Chem Phys Lipids. 2020 Jan;226:104837
pubmed: 31689410
Nature. 2016 Jun 01;534(7607):396-401
pubmed: 27281205
Am J Respir Crit Care Med. 2018 Jan 1;197(1):104-116
pubmed: 28853608
Angew Chem Int Ed Engl. 2012 Aug 20;51(34):8529-33
pubmed: 22782619
Nat Methods. 2008 May;5(5):439-45
pubmed: 18391960
Cell. 2009 Oct 16;139(2):285-98
pubmed: 19837032
Circ Res. 2010 Oct 15;107(8):943-52
pubmed: 20947863
Circulation. 2004 Jun 8;109(22):2692-7
pubmed: 15184293
J Cell Sci. 2016 Jun 1;129(11):2182-9
pubmed: 27252357
Sci Rep. 2018 Jan 23;8(1):1419
pubmed: 29362496
Nucleic Acids Res. 2010 Jan;38(1):215-24
pubmed: 19850715
Mol Ther Nucleic Acids. 2012 Aug 14;1:e37
pubmed: 23344179
Circ Res. 2017 Apr 28;120(9):1466-1476
pubmed: 28298297
ACS Nano. 2015 Jul 28;9(7):6706-16
pubmed: 26042619
Nat Biotechnol. 2010 Feb;28(2):172-6
pubmed: 20081866
Oncogene. 2010 May 20;29(20):3010-6
pubmed: 20208559
Oncotarget. 2017 Jul 11;8(28):45200-45212
pubmed: 28423355
Front Cell Dev Biol. 2020 May 25;8:367
pubmed: 32528952
Cells. 2020 Mar 10;9(3):
pubmed: 32164322
ACS Nano. 2017 Aug 22;11(8):7572-7586
pubmed: 28727419
Adv Genet. 2014;88:71-110
pubmed: 25409604
Mol Ther. 2017 Mar 1;25(3):679-693
pubmed: 28159509
J Vis Exp. 2007;(3):186
pubmed: 18978935
Brain Behav Immun. 2020 Jul;87:543-555
pubmed: 32017988
Stem Cell Res. 2007 Nov;1(2):129-37
pubmed: 19383393
Acta Cardiol Sin. 2014 Sep;30(5):395-400
pubmed: 27122816
Stem Cell Res. 2013 May;10(3):301-12
pubmed: 23399448
Circulation. 2004 Jun 1;109(21):2487-91
pubmed: 15173038
N Engl J Med. 2018 Jul 5;379(1):11-21
pubmed: 29972753
Cells. 2020 Mar 09;9(3):
pubmed: 32182815
Nat Rev Cardiol. 2017 May;14(5):259-272
pubmed: 28150804
Biochimie. 2017 Apr;135:111-125
pubmed: 28192157
Adv Mater. 2020 Mar;32(12):e1906128
pubmed: 31999380
Proc Natl Acad Sci U S A. 2016 Jan 5;113(1):E16-22
pubmed: 26699502
J Exp Med. 2012 Aug 27;209(9):1611-27
pubmed: 22908324
Circ Res. 2011 Sep 30;109(8):894-906
pubmed: 21868695
Circ Res. 2015 Jun 19;117(1):7-9
pubmed: 26089361
J Cell Sci. 2000 Oct;113 Pt 19:3365-74
pubmed: 10984428