Digital Image Analysis has an Additive Beneficial Role to Conventional Cytology in Diagnosing the Nature of Biliary Ducts Stricture.

CCE, conventional cytological evaluation DIA, digital image analysis DNA image analysis FISH, fluorescence in-situ hybridization IBDS, indeterminate biliary duct stricture IDUS, intraductal ultrasound MRCP, magnetic resonance cholangiopancreatography biliary malignancy conventional cytological evaluation diagnostic accuracy indeterminate biliary duct stricture

Journal

Journal of clinical and experimental hepatology
ISSN: 0973-6883
Titre abrégé: J Clin Exp Hepatol
Pays: India
ID NLM: 101574137

Informations de publication

Date de publication:
Historique:
received: 15 03 2020
accepted: 18 07 2020
entrez: 22 3 2021
pubmed: 23 3 2021
medline: 23 3 2021
Statut: ppublish

Résumé

Conventional cytological evaluation (CCE) fails to identify nature indeterminate biliary duct stricture (IBDS) in many cases. Digital image analysis (DIA) has the ability to identify and analyze the DNA content of cells. This study assesses the role of DIA in recognizing the nature of IBDS compared to CCE. A prospective observational study was conducted at the Al-Rajhi University Hospital. Fifty patients with IBDS, based on abdominal imaging, were subjected to endoscopic retrograde cholangiopancreatography (ERCP) and brush sampling. These samples were evaluated with CCE and DIA. Follow-up for at least 9 months and cost-analysis had also been done. Based on the final diagnosis, 32 (64.0%) patients had malignant stricture, and 39 (78.0%) had distal stricture. DIA had 84.40% (95% CI; 67.20-94.70) sensitivity and 94.40% (95% CI; 72.70-99.90) specificity in identifying nature of IBDS, whereas CCE had 19.0% (95% CI; 7.20-36.40) sensitivity and 89.0% (95% CI; 65.30-98.60) specificity. Combination of both modalities had 84.40% (95% CI; 67.20-94.70) sensitivity and 83.30% (95% CI; 58.60-96.40) specificity in identification nature of IBDS. Based on CCE alone, only 6/32 (18.80%) of malignant stricture were diagnosed, and 26/32 (81.20%) were missed. However, DIA alone was able to diagnose 27/32 (84.40%) of malignant stricture, and only 5 cases were missed. Both procedures had detection rate of malignant stricture as DIA alone. Benign stricture was correctly diagnosed in 16/18 (88.80%), 17/18 (94.40%), and 15/18 (83.30%) using CCE alone, DIA alone, and both procedures together, respectively. Cost per detection additional one malignant stricture using DIA required 99.4$. DIA is substantially better than CCE in diagnosing the nature of IBDS but at an increase cost and thus suggests its application in a wider role in clinical practice. NCT04112030.

Sections du résumé

BACKGROUND & AIM OBJECTIVE
Conventional cytological evaluation (CCE) fails to identify nature indeterminate biliary duct stricture (IBDS) in many cases. Digital image analysis (DIA) has the ability to identify and analyze the DNA content of cells. This study assesses the role of DIA in recognizing the nature of IBDS compared to CCE.
METHODS METHODS
A prospective observational study was conducted at the Al-Rajhi University Hospital. Fifty patients with IBDS, based on abdominal imaging, were subjected to endoscopic retrograde cholangiopancreatography (ERCP) and brush sampling. These samples were evaluated with CCE and DIA. Follow-up for at least 9 months and cost-analysis had also been done.
RESULTS RESULTS
Based on the final diagnosis, 32 (64.0%) patients had malignant stricture, and 39 (78.0%) had distal stricture. DIA had 84.40% (95% CI; 67.20-94.70) sensitivity and 94.40% (95% CI; 72.70-99.90) specificity in identifying nature of IBDS, whereas CCE had 19.0% (95% CI; 7.20-36.40) sensitivity and 89.0% (95% CI; 65.30-98.60) specificity. Combination of both modalities had 84.40% (95% CI; 67.20-94.70) sensitivity and 83.30% (95% CI; 58.60-96.40) specificity in identification nature of IBDS. Based on CCE alone, only 6/32 (18.80%) of malignant stricture were diagnosed, and 26/32 (81.20%) were missed. However, DIA alone was able to diagnose 27/32 (84.40%) of malignant stricture, and only 5 cases were missed. Both procedures had detection rate of malignant stricture as DIA alone. Benign stricture was correctly diagnosed in 16/18 (88.80%), 17/18 (94.40%), and 15/18 (83.30%) using CCE alone, DIA alone, and both procedures together, respectively. Cost per detection additional one malignant stricture using DIA required 99.4$.
CONCLUSION CONCLUSIONS
DIA is substantially better than CCE in diagnosing the nature of IBDS but at an increase cost and thus suggests its application in a wider role in clinical practice.
CLINICAL TRIAL NUMBER BACKGROUND
NCT04112030.

Identifiants

pubmed: 33746446
doi: 10.1016/j.jceh.2020.07.009
pii: S0973-6883(20)30109-2
pmc: PMC7953004
doi:

Banques de données

ClinicalTrials.gov
['NCT04112030']

Types de publication

Journal Article

Langues

eng

Pagination

209-218

Informations de copyright

© 2020 Indian National Association for Study of the Liver. Published by Elsevier B.V. All rights reserved.

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Auteurs

Ahmed Helmy (A)

Department of Tropical Medicine and Gastroenterology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Heba Mohamed Saad Eldien (HM)

Department of Histology and Cell Biology, Faculty of Medicine, Assiut University, Assiut, Egypt.
Tissue Culture and Molecular Biology Center, Assiut University, Egypt.
Department of Anatomy College of Medicine, Jouf University, KSA.

Gehan Sayed Seifeldein (GS)

Department of Diagnostic and Interventional Radiology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Ahmed Mohammed Abu-Elfatth (AM)

Department of Tropical Medicine and Gastroenterology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Adnan Ahmed Mohammed (AA)

Department of Tropical Medicine and Gastroenterology, Faculty of Medicine, Assiut University, Assiut, Egypt.

Classifications MeSH