Comparative intravital imaging of human and rodent malaria sporozoites reveals the skin is not a species-specific barrier.
Plasmodium
intravital
malaria
skin
sporozoites
Journal
EMBO molecular medicine
ISSN: 1757-4684
Titre abrégé: EMBO Mol Med
Pays: England
ID NLM: 101487380
Informations de publication
Date de publication:
09 04 2021
09 04 2021
Historique:
revised:
21
01
2021
received:
20
11
2019
accepted:
27
01
2021
pubmed:
23
3
2021
medline:
26
10
2021
entrez:
22
3
2021
Statut:
ppublish
Résumé
Malaria infection starts with the injection of Plasmodium sporozoites into the host's skin. Sporozoites are motile and move in the skin to find and enter blood vessels to be carried to the liver. Here, we present the first characterization of P. falciparum sporozoites in vivo, analyzing their motility in mouse skin and human skin xenografts and comparing their motility to two rodent malaria species. These data suggest that in contrast to the liver and blood stages, the skin is not a species-specific barrier for Plasmodium. Indeed, P. falciparum sporozoites enter blood vessels in mouse skin at similar rates to the rodent malaria parasites. Furthermore, we demonstrate that antibodies targeting sporozoites significantly impact the motility of P. falciparum sporozoites in mouse skin. Though the sporozoite stage is a validated vaccine target, vaccine trials have been hampered by the lack of good animal models for human malaria parasites. Pre-clinical screening of next-generation vaccines would be significantly aided by the in vivo platform we describe here, expediting down-selection of candidates prior to human vaccine trials.
Identifiants
pubmed: 33750026
doi: 10.15252/emmm.201911796
pmc: PMC8033530
doi:
Substances chimiques
Malaria Vaccines
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e11796Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR073665
Pays : United States
Organisme : NIAMS NIH HHS
ID : K01 AR073924
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR069502
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI132359
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021 The Authors. Published under the terms of the CC BY 4.0 license.
Références
Curr Opin Microbiol. 2016 Aug;32:96-102
pubmed: 27262417
J Exp Med. 2000 Oct 16;192(8):1205-11
pubmed: 11034611
Malar J. 2016 Apr 30;15(1):251
pubmed: 27129682
Infect Immun. 2005 Jul;73(7):4363-9
pubmed: 15972531
Nat Protoc. 2007;2(7):1705-12
pubmed: 17641635
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
Annu Rev Immunol. 2019 Apr 26;37:225-246
pubmed: 30566366
mBio. 2018 Nov 20;9(6):
pubmed: 30459199
J Immunol. 1984 Feb;132(2):909-13
pubmed: 6317752
Clin Vaccine Immunol. 2013 Jun;20(6):803-10
pubmed: 23536694
Infect Immun. 1991 Aug;59(8):2529-34
pubmed: 1855974
J Clin Invest. 2014 Jan;124(1):140-4
pubmed: 24292709
Semin Immunopathol. 2012 Nov;34(6):787-92
pubmed: 23053392
PLoS Pathog. 2011 Jun;7(6):e1002080
pubmed: 21698220
PLoS Pathog. 2012;8(7):e1002725
pubmed: 22911675
Nat Genet. 2005 Jul;37(7):745-9
pubmed: 15951821
Parasitol Int. 2014 Feb;63(1):237-44
pubmed: 23892177
PLoS One. 2011;6(12):e29289
pubmed: 22216235
Mol Biochem Parasitol. 1996 Apr;77(1):7-17
pubmed: 8784767
Nat Med. 2006 Feb;12(2):220-4
pubmed: 16429144
J Exp Med. 2005 Jan 3;201(1):27-33
pubmed: 15630135
Malar J. 2016 Apr 12;15:201
pubmed: 27068454
Infect Immun. 2013 Aug;81(8):2882-7
pubmed: 23716612
Science. 1991 May 3;252(5006):715-8
pubmed: 1827210
Malar J. 2018 Aug 31;17(1):319
pubmed: 30170589
J Exp Med. 1982 Jul 1;156(1):20-30
pubmed: 7045272
Immunol Lett. 1990 Aug;25(1-3):39-42
pubmed: 2283161
NPJ Vaccines. 2017 Oct 9;2:27
pubmed: 29263882
EMBO Mol Med. 2021 Apr 9;13(4):e11796
pubmed: 33750026
Nat Rev Immunol. 2009 Nov;9(11):789-98
pubmed: 19834485
J Dermatol Sci. 2018 Apr;90(1):46-51
pubmed: 29352630
Nat Immunol. 2018 Nov;19(11):1199-1211
pubmed: 30333613
Int J Parasitol. 2007 Feb;37(2):173-82
pubmed: 17112526
Int J Parasitol. 2004 Aug;34(9):991-6
pubmed: 15313126
PLoS Med. 2014 Jul 29;11(7):e1001685
pubmed: 25072396
Elife. 2015 Aug 13;4:
pubmed: 26271010
J Exp Med. 2011 Feb 14;208(2):341-56
pubmed: 21262960
Malar J. 2012 Mar 31;11:103
pubmed: 22463060
Differentiation. 2017 Nov - Dec;98:14-24
pubmed: 29059535
Nat Microbiol. 2018 Nov;3(11):1224-1233
pubmed: 30349082
Cell Microbiol. 2007 May;9(5):1215-22
pubmed: 17223931
Nat Med. 2018 May;24(4):408-416
pubmed: 29554083
Sci Rep. 2019 Sep 11;9(1):13131
pubmed: 31511546
J Protozool. 1974 Oct;21(4):527-37
pubmed: 4138523
Cell Microbiol. 2014 May;16(5):734-50
pubmed: 24612056
Ann N Y Acad Sci. 2015 Apr;1342:37-43
pubmed: 25694058
J Invest Dermatol. 2020 Aug;140(8):1488-1497.e1
pubmed: 32407714
Nat Protoc. 2006;1(1):346-56
pubmed: 17406255
Front Immunol. 2018 Apr 19;9:807
pubmed: 29725334
Sci Rep. 2019 Sep 17;9(1):13436
pubmed: 31530862
Nat Methods. 2012 Jun 28;9(7):690-6
pubmed: 22743774
Science. 2010 Aug 13;329(5993):856-61
pubmed: 20616233
Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):9146-50
pubmed: 8090783