Development of a hollow fibre-based renal module for active transport studies.
3D cell culture
Drug transport
Fluidic shear stress
Hollow fibre
Renal function
Journal
Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs
ISSN: 1619-0904
Titre abrégé: J Artif Organs
Pays: Japan
ID NLM: 9815648
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
12
10
2020
accepted:
10
03
2021
pubmed:
23
3
2021
medline:
10
11
2021
entrez:
22
3
2021
Statut:
ppublish
Résumé
Understanding the active transport of substrates by the kidney in the renal proximal convoluted tubule is crucial for drug development and for studying kidney diseases. Currently, cell-based assays are applied for this this purpose, however, differences between assays and the body are common, indicating the importance of in vitro-in vivo discrepancies. Several studies have suggested that 3D cell cultures expose cells to a more physiological environments, thus, providing more accurate cell function results. To mimic the renal proximal tubule, we have developed a custom-made renal module (RM), containing a single polypropylene hollow fibre (Plasmaphan P1LX, 3M) that serves as a porous scaffold and compared to conventional Transwell cell-based bidirectional transport studies. In addition, a constant flow of media, exposed cells to a physiological shear stress of 0.2 dyne/cm
Identifiants
pubmed: 33751266
doi: 10.1007/s10047-021-01260-w
pii: 10.1007/s10047-021-01260-w
pmc: PMC8571221
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
473-484Informations de copyright
© 2021. The Author(s).
Références
Kidney Int. 2006 Feb;69(3):440-9
pubmed: 16514429
J Biol Chem. 2000 Feb 25;275(8):5512-20
pubmed: 10681530
Kidney Int. 2004 Oct;66(4):1578-88
pubmed: 15458454
Mol Pharm. 2016 Mar 7;13(3):933-44
pubmed: 26871298
PLoS One. 2013;8(3):e57227
pubmed: 23505419
Biofabrication. 2016 Mar 24;8(1):015021
pubmed: 27011358
J Cell Sci. 2004 Aug 15;117(Pt 18):4301-11
pubmed: 15316084
Eur J Pharmacol. 2016 Nov 5;790:28-35
pubmed: 27395800
Biomaterials. 2009 May;30(15):2899-911
pubmed: 19217158
Biotechnol Bioeng. 2012 Mar;109(3):797-803
pubmed: 22012446
Clin J Am Soc Nephrol. 2015 Nov 6;10(11):2039-49
pubmed: 26490509
Kidney Int. 1999 Jun;55(6):2502-14
pubmed: 10354300
Acta Biomater. 2015 Mar;14:22-32
pubmed: 25527093
Int J Biomater. 2013;2013:620765
pubmed: 24222766
Nat Biotechnol. 1999 May;17(5):451-5
pubmed: 10331803
Pharmacotherapy. 2003 Apr;23(4):436-42
pubmed: 12680473
Analyst. 2014 Jul 7;139(13):3206-18
pubmed: 24668405
AAPS J. 2018 Jul 26;20(5):87
pubmed: 30051196
Biotechnol Adv. 2014 Nov 15;32(7):1317-1327
pubmed: 25135479
J Pharm Sci. 2004 May;93(5):1185-94
pubmed: 15067695
Life Support Syst. 1987 Apr-Jun;5(2):159-68
pubmed: 3669723
Sci Rep. 2015 Nov 16;5:16702
pubmed: 26567716
Front Physiol. 2017 Mar 08;8:123
pubmed: 28337147
Medchemcomm. 2016 Jun 1;7(6):1069-1081
pubmed: 27672436
Biotechniques. 2007 Nov;43(5):575, 577-8, 581-2 passim
pubmed: 18072586
Curr Top Membr. 2014;73:233-61
pubmed: 24745985
AAPS J. 2018 Aug 14;20(5):90
pubmed: 30109442
Molecules. 2020 Jan 29;25(3):
pubmed: 32013171
Drug Discov Today. 2013 Oct;18(19-20):922-35
pubmed: 23748137
J Biol Chem. 2002 Oct 18;277(42):39739-48
pubmed: 12138159
Nat Biotechnol. 2014 Aug;32(8):760-72
pubmed: 25093883
J Health Econ. 2003 Mar;22(2):151-85
pubmed: 12606142
Kidney Int. 1999 Dec;56(6):2016-24
pubmed: 10594777
Biochim Biophys Acta Gen Subj. 2020 Jan;1864(1):129433
pubmed: 31520681
Curr Protoc Cell Biol. 2016 Sep 01;72:10.20.1-10.20.16
pubmed: 27580704
Integr Biol (Camb). 2013 Sep;5(9):1119-29
pubmed: 23644926
Sci Rep. 2016 Oct 11;6:34845
pubmed: 27725720
Proc Natl Acad Sci U S A. 2014 Jun 10;111(23):8506-11
pubmed: 24912170
Proc Natl Acad Sci U S A. 2008 Aug 12;105(32):11418-23
pubmed: 18685100
Kidney Int. 1994 Jan;45(1):48-57
pubmed: 8127021
Biomaterials. 2006 Jun;27(16):3096-108
pubmed: 16460797
Biomaterials. 2011 Feb;32(6):1465-76
pubmed: 21145586
J Pharmacol Exp Ther. 2019 Jun;369(3):523-530
pubmed: 30910922
IUBMB Life. 2013 Dec;65(12):991-8
pubmed: 24323927