Mass transfer into biological matter using isochoric freezing.

Cold Temperature Cryopreservation / methods Cryoprotective Agents Freezing Isochores
Cryoprotectants Isochoric freezing Mass transfer

Journal

Cryobiology
ISSN: 1090-2392
Titre abrégé: Cryobiology
Pays: Netherlands
ID NLM: 0006252

Informations de publication

Date de publication:
06 2021
Historique:
received: 07 09 2020
revised: 12 02 2021
accepted: 16 03 2021
pubmed: 25 3 2021
medline: 29 6 2021
entrez: 24 3 2021
Statut: ppublish

Résumé

This paper is a theoretical study of a protocol for transport of high concentrations of cryoprotectants into biological matter, using isochoric freezing. Unlike isobaric freezing, where the entire system freezes at temperatures lower than the freezing temperature, in isochoric freezing a substantial portion of the system remains unfrozen at temperatures below freezing. In isochoric freezing cryopreservation, the system is designed in such a way that the biological matter remains unfrozen and surrounded by an unfrozen solution. The protocol in this study involves the freezing of an isochoric systems along the "liquidus line" at which water and ice are in thermodynamic equilibrium. Rejection of solutes by ice increases the concentration of the solutes in the unfrozen solution surrounding the unfrozen biological matter, leading, thereby, to transport of increasingly higher concentrations of cryoprotectants into the biological matter, as the temperature of the system is lowered and the toxicity of the cryoprotectants is reduced.

Identifiants

DOI: 10.1016/j.cryobiol.2021.03.004 PMID: 33757760
pubmed: 33757760
pii: S0011-2240(21)00064-X
doi: 10.1016/j.cryobiol.2021.03.004
pii:
doi:

Substances chimiques

Cryoprotective Agents 0
Isochores 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

212-215

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Boris Rubinsky (B)

Department of Mechanical Engineering, Department of Bioengineering, University of California Berkeley, 94720, Berkeley, CA, USA. Electronic address: rubinsky@berkeley.edu.

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Classifications MeSH

questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Basse température › Mass transfer into biological matter using...
questionsmedicales.fr › Techniques d'investigation › Conservation biologique › Cryoconservation › Mass transfer into biological matter using...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Agents protecteurs › Cryoprotecteurs › Mass transfer into biological matter using...
questionsmedicales.fr › Phénomènes chimiques › Transition de phase › Congélation › Mass transfer into biological matter using...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Isochores › Mass transfer into biological matter using...