Memory B cell repertoire for recognition of evolving SARS-CoV-2 spike.

Journal

bioRxiv : the preprint server for biology
Titre abrégé: bioRxiv
Pays: United States
ID NLM: 101680187

Informations de publication

Date de publication:
10 Mar 2021
Historique:
pubmed: 25 3 2021
medline: 25 3 2021
entrez: 24 3 2021
Statut: epublish

Résumé

Memory B cell reserves can generate protective antibodies against repeated SARS-CoV-2 infections, but with an unknown reach from original infection to antigenically drifted variants. We charted memory B cell receptor-encoded monoclonal antibodies (mAbs) from 19 COVID-19 convalescent subjects against SARS-CoV-2 spike (S) and found 7 major mAb competition groups against epitopes recurrently targeted across individuals. Inclusion of published and newly determined structures of mAb-S complexes identified corresponding epitopic regions. Group assignment correlated with cross-CoV-reactivity breadth, neutralization potency, and convergent antibody signatures. mAbs that competed for binding the original S isolate bound differentially to S variants, suggesting the protective importance of otherwise-redundant recognition. The results furnish a global atlas of the S-specific memory B cell repertoire and illustrate properties conferring robustness against emerging SARS-CoV-2 variants.

Identifiants

DOI: 10.1101/2021.03.10.434840 PMID: 33758863 PMC: PMC7987022
pubmed: 33758863
doi: 10.1101/2021.03.10.434840
pmc: PMC7987022
pii:
doi:

Types de publication

Preprint

Langues

eng

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI146779
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI147884
Pays : United States
Organisme : NIAID NIH HHS
ID : T32 AI007245
Pays : United States

Commentaires et corrections

Type : UpdateIn

Déclaration de conflit d'intérêts

Competing interests The authors declare no competing interests.

Auteurs

Pei Tong (P)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Avneesh Gautam (A)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Ian Windsor (I)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Meghan Travers (M)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Yuezhou Chen (Y)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Nicholas Garcia (N)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Noah B Whiteman (NB)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Lindsay G A McKay (LGA)

Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA.
National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA.

Felipe J N Lelis (FJN)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Shaghayegh Habibi (S)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Yongfei Cai (Y)

Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.

Linda J Rennick (LJ)

The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.
The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh PA.

W Paul Duprex (WP)

The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.
The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh PA.

Kevin R McCarthy (KR)

The Center for Vaccine Research, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261.
The Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh PA.

Christy L Lavine (CL)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.

Teng Zuo (T)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Junrui Lin (J)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Adam Zuiani (A)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.

Jared Feldman (J)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

Elizabeth A MacDonald (EA)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.

Blake M Hauser (BM)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.

Anthony Griffths (A)

Department of Microbiology, Boston University School of Medicine, Boston, MA 02115, USA.
National Emerging Infectious Diseases Laboratories, Boston University, Boston, MA 02115, USA.

Michael S Seaman (MS)

Center for Virology and Vaccine Research, Beth Israel Deaconess Medical Center, Boston, MA 02115, USA.

Aaron G Schmidt (AG)

Ragon Institute of MGH, MIT, and Harvard, Cambridge, MA 02139, USA.
Department of Microbiology, Harvard Medical School, Boston MA 02115.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA.

Bing Chen (B)

Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA.

Donna Neuberg (D)

Department of Data Science, Dana-Farber Cancer Institute, Boston, MA 02215, USA.

Goran Bajic (G)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.

Stephen C Harrison (SC)

Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA.
Laboratory of Molecular Medicine, Boston Children's Hospital, Boston, MA 02115, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA.
Howard Hughes Medical Institute, Boston, MA 02115, USA.

Duane R Wesemann (DR)

Department of Medicine, Division of Allergy and Immunology, Division of Genetics, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
Massachusetts Consortium on Pathogen Readiness, Boston, MA 02115, USA.

Classifications MeSH