Integrating high cell density cultures with adapted laboratory evolution for improved Gag-HA virus-like particles production in stable insect cell lines.
adaptive laboratory evolution
high cell density
influenza virus-like particles
insect cells
perfusion
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
07 2021
07 2021
Historique:
revised:
05
03
2021
received:
14
01
2021
accepted:
23
03
2021
pubmed:
26
3
2021
medline:
18
1
2022
entrez:
25
3
2021
Statut:
ppublish
Résumé
Stable insect cell lines are emerging as an alternative to the insect cell-baculovirus expression vector system (IC-BEVS) for protein expression, benefiting from being a virus-free, nonlytic system. Still, the titers achieved are considerably lower. In this study, stable insect (Sf-9 and High Five) cells producing Gag virus-like particles (VLPs) were first adapted to grow under hypothermic culture conditions (22°C instead of standard 27°C), and then pseudotyped with a model membrane protein (influenza hemagglutinin [HA]) for expression of Gag-HA VLPs. Adaptation to lower temperature led to an increase in protein titers of up to 12-fold for p24 (as proxy for Gag-VLP) and sixfold for HA, with adapted Sf-9 cells outperforming High Five cells. Resulting Gag-HA VLPs producer Sf-9 cells were cultured to high cell densities, that is, 100 × 10
Substances chimiques
HIV Core Protein p24
0
Hemagglutinin Glycoproteins, Influenza Virus
0
Recombinant Fusion Proteins
0
p24 protein, Human Immunodeficiency Virus Type 1
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2536-2547Informations de copyright
© 2021 Wiley Periodicals LLC.
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