Helicobacter pylori Virulence Factor Genotyping.
Agarose gel electrophoresis
DNA extraction
Helicobacter pylori
PCR
Virulence factor
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
25
3
2021
pubmed:
26
3
2021
medline:
12
6
2021
Statut:
ppublish
Résumé
Helicobacter pylori (H. pylori) infection causes chronic gastritis, peptic ulcers, gastric adenocarcinoma, and mucosa-associated lymphoid tissue (MALT) lymphoma. Bacterial, host, and environmental factors influence the progression of disease from superficial gastritis to cancer. H. pylori is genetically diverse, and expression of its specific virulence factors has been linked to increased risk of more severe pathologies. Described in this chapter is a protocol for detecting important H. pylori virulence factors by firstly extracting DNA from culture material or stomach tissue biopsies, followed by PCR amplification and agarose gel electrophoresis.
Identifiants
pubmed: 33765313
doi: 10.1007/978-1-0716-1302-3_11
doi:
Substances chimiques
Bacterial Proteins
0
Virulence Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
93-106Références
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