Uptake of polymeric nanoparticles in a human induced pluripotent stem cell-based blood-brain barrier model: Impact of size, material, and protein corona.
Blood-Brain Barrier
/ metabolism
Calibration
Cell Differentiation
Dynamic Light Scattering
Electric Impedance
Endothelial Cells
/ metabolism
Fractionation, Field Flow
Humans
Induced Pluripotent Stem Cells
/ metabolism
Models, Biological
Nanoparticles
/ chemistry
Particle Size
Polylactic Acid-Polyglycolic Acid Copolymer
/ chemistry
Polymers
/ chemistry
Polystyrenes
/ chemistry
Protein Corona
/ chemistry
Static Electricity
Journal
Biointerphases
ISSN: 1559-4106
Titre abrégé: Biointerphases
Pays: United States
ID NLM: 101275679
Informations de publication
Date de publication:
25 03 2021
25 03 2021
Historique:
entrez:
26
3
2021
pubmed:
27
3
2021
medline:
25
12
2021
Statut:
epublish
Résumé
The blood-brain barrier (BBB) maintains the homeostasis of the central nervous system, which is one of the reasons for the treatments of brain disorders being challenging in nature. Nanoparticles (NPs) have been seen as potential drug delivery systems to the brain overcoming the tight barrier of endothelial cells. Using a BBB model system based on human induced pluripotent stem cells (iPSCs), the impact of polymeric nanoparticles has been studied in relation to nanoparticle size, material, and protein corona. PLGA [poly(lactic-co-glycolic acid)] and PLLA [poly(d,l-lactide)] nanoparticles stabilized with Tween® 80 were synthesized (50 and 100 nm). iPSCs were differentiated into human brain microvascular endothelial cells (hBMECs), which express prominent BBB features, and a tight barrier was established with a high transendothelial electrical resistance of up to 4000 Ω cm
Substances chimiques
Polymers
0
Polystyrenes
0
Protein Corona
0
Polylactic Acid-Polyglycolic Acid Copolymer
1SIA8062RS
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM