The Burkholderia pseudomallei intracellular 'TRANSITome'.
Animals
Bacterial Proteins
/ genetics
Burkholderia pseudomallei
/ cytology
Cell Line, Tumor
Cell Membrane
/ microbiology
Computational Biology
/ methods
Cytoplasm
/ microbiology
Gene Expression Profiling
/ methods
Genes, Bacterial
/ genetics
HEK293 Cells
Host-Pathogen Interactions
Humans
Melioidosis
/ microbiology
Mice
Mice, Inbred BALB C
RAW 264.7 Cells
Single-Cell Analysis
/ methods
Vacuoles
/ microbiology
Virulence
/ genetics
Virulence Factors
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 03 2021
26 03 2021
Historique:
received:
10
08
2020
accepted:
26
02
2021
entrez:
27
3
2021
pubmed:
28
3
2021
medline:
13
4
2021
Statut:
epublish
Résumé
Prokaryotic cell transcriptomics has been limited to mixed or sub-population dynamics and individual cells within heterogeneous populations, which has hampered further understanding of spatiotemporal and stage-specific processes of prokaryotic cells within complex environments. Here we develop a 'TRANSITomic' approach to profile transcriptomes of single Burkholderia pseudomallei cells as they transit through host cell infection at defined stages, yielding pathophysiological insights. We find that B. pseudomallei transits through host cells during infection in three observable stages: vacuole entry; cytoplasmic escape and replication; and membrane protrusion, promoting cell-to-cell spread. The B. pseudomallei 'TRANSITome' reveals dynamic gene-expression flux during transit in host cells and identifies genes that are required for pathogenesis. We find several hypothetical proteins and assign them to virulence mechanisms, including attachment, cytoskeletal modulation, and autophagy evasion. The B. pseudomallei 'TRANSITome' provides prokaryotic single-cell transcriptomics information enabling high-resolution understanding of host-pathogen interactions.
Identifiants
pubmed: 33772012
doi: 10.1038/s41467-021-22169-1
pii: 10.1038/s41467-021-22169-1
pmc: PMC7998038
doi:
Substances chimiques
Bacterial Proteins
0
Virulence Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1907Subventions
Organisme : NIGMS NIH HHS
ID : P30 GM114737
Pays : United States
Organisme : NIAID NIH HHS
ID : U54 AI065359
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM103580
Pays : United States
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