Complement regulation in tenocytes under the influence of leukocytes in an indirect co-culture model.
Complement regulation
Complement system
Indirect co-culture
Leukocytes
TNFα
Tendinopathy
Tenocytes
Journal
Inflammation research : official journal of the European Histamine Research Society ... [et al.]
ISSN: 1420-908X
Titre abrégé: Inflamm Res
Pays: Switzerland
ID NLM: 9508160
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
05
03
2019
accepted:
09
01
2020
revised:
31
12
2019
pubmed:
28
3
2021
medline:
6
11
2021
entrez:
27
3
2021
Statut:
ppublish
Résumé
The present in vitro study was undertaken to learn about the effects of leukocytes on tenocytes in respect to complement regulation simulating an inflammatory scenario of the traumatized tissue. Human hamstring tendon-derived tenocyte monolayers were co-cultured indirectly with human leukocytes (either Peripheral Blood Mononuclear Cells [PBMCs] or neutrophils) using a transwell system with/without (+ / C5aR gene expression was significantly induced by TNFα after 4 h, but impaired in the presence of leukocytes + TNFα after 24 h. At 4 h, PBMCs activated by TNFα induced the CRP CD46 gene expression. However, CD55 was significantly suppressed after 24 h by neutrophils + / TNFα might represent a crucial soluble mediator exerting diverse time-dependent effects on tenocyte complement regulation.
Identifiants
pubmed: 33772629
doi: 10.1007/s00011-021-01451-4
pii: 10.1007/s00011-021-01451-4
doi:
Substances chimiques
Antigens, CD
0
C5AR1 protein, human
0
Receptor, Anaphylatoxin C5a
0
Tumor Necrosis Factor-alpha
0
Complement System Proteins
9007-36-7
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
495-507Références
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