Highly sensitive electrochemical detection of the marine toxins okadaic acid and domoic acid with carbon black modified screen printed electrodes.
Carbon black
Electrochemistry
Immunoassay
Immunosensor
Pulsed voltammetry
Screen printed electrode
Toxin
biosensor
Journal
Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R
Informations de publication
Date de publication:
01 Jun 2021
01 Jun 2021
Historique:
received:
08
12
2020
revised:
07
02
2021
accepted:
11
02
2021
entrez:
28
3
2021
pubmed:
29
3
2021
medline:
15
5
2021
Statut:
ppublish
Résumé
A novel electrochemical immunosensor for the detection of the important marine biotoxins domoic acid (DA) and okadaic acid (OA) was developed. The sensors used carbon black modified screen-printed electrodes (CB-SPE) obtained using a high-throughput method. The electrochemical performance and stability of CB modified SPEs and bare carbon SPEs (c-SPEs) were compared using cyclic voltammetry and electrochemical impedance spectroscopy. CB-SPEs showed improved long-term (at least six months) stability and electro-catalytic properties compared with c-SPEs. The CB-SPEs were bio-functionalized with DA or OA protein-conjugates and used to develop two indirect competitive immunosensors using differential pulse voltammetry (DPV). The DPV signals obtained for the OA and DA immunosensors fitted well to four-parameter dose-response curves (R
Identifiants
pubmed: 33773701
pii: S0039-9140(21)00136-3
doi: 10.1016/j.talanta.2021.122215
pii:
doi:
Substances chimiques
Marine Toxins
0
Soot
0
Okadaic Acid
1W21G5Q4N2
domoic acid
M02525818H
Kainic Acid
SIV03811UC
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
122215Informations de copyright
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