Expression of Phosphodiesterase (PDE) Isoenzymes in the Human Male and Female Urethra.
cyclic adenosine monophosphate
nitric oxide/cyclic guanosine monophosphate
phosphodiesterase isoenzymes
urethral smooth muscle
Journal
Research and reports in urology
ISSN: 2253-2447
Titre abrégé: Res Rep Urol
Pays: England
ID NLM: 101576971
Informations de publication
Date de publication:
2021
2021
Historique:
received:
26
11
2020
accepted:
18
02
2021
entrez:
29
3
2021
pubmed:
30
3
2021
medline:
30
3
2021
Statut:
epublish
Résumé
Although it has been supposed that the NO/cyclic GMP system produces inhibitory signals to reduce the resistance of the bladder outlet and urethra during the micturition phase, little is known on the mechanisms controlling the function of urethral smooth muscle. The aim of the present study was to examine in the male and female urethra the expression of phosphodiesterase (PDE) isoenzymes, known as key proteins of the cyclic GMP/AMP signaling. Urethral tissue was obtained from 4 female cadavers and 7 male patients (who had undergone gender reassignment surgery). The expression of mRNA encoding for PDE1A, 1B, 1C, 2A, 4B, 4D, 5A, 10A and 11A was investigated by means of real-time polymerase chain reaction. Western blot (WB) analysis was conducted to detect PDE isoenzymes. RT-PCR revealed relevant amounts of mRNA encoding for PDE1A, 2A, 4B, 5A, 10A and 11A in male and female urethral tissue. The expression of PDE1A, 2A, 4B and 10A was 2-fold higher in the female than in the male urethra, whereas the expression of PDE11A mRNA was 7-fold higher in the male tissue. In the WB experiments, immunosignals specific for PDE1A, PDE4A and 4B and PDE11A were of higher degree in the female than the male tissue specimens, while an almost equivocal expression of PDE2A, PDE5A and PDE10A was registered. On the level of mRNA and function proteins, different patterns of expression of PDE isoenzymes were registered in human male and female urethra. Future studies may clarify whether inhibition of PDE isoenzymes is likely to facilitate the relaxation of the outflow region in both sexes.
Identifiants
pubmed: 33777854
doi: 10.2147/RRU.S291962
pii: 291962
pmc: PMC7989682
doi:
Types de publication
Journal Article
Langues
eng
Pagination
139-145Informations de copyright
© 2021 Rahardjo et al.
Déclaration de conflit d'intérêts
The authors have no conflicts of interest in this work.
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