Variation of apoptotic and proliferative activity among lymphoma subtypes in dogs: A flow cytometric study.
Annexin V
Ki67
Proliferation/apoptosis ratio
Turnover index
Journal
Research in veterinary science
ISSN: 1532-2661
Titre abrégé: Res Vet Sci
Pays: England
ID NLM: 0401300
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
28
06
2020
revised:
10
10
2020
accepted:
14
10
2020
pubmed:
30
3
2021
medline:
29
4
2021
entrez:
29
3
2021
Statut:
ppublish
Résumé
Tumor growth depends on both proliferative and apoptotic rate of neoplastic cells. High proliferation index is a well-known negative prognostic factor in canine lymphomas, whereas little is known about apoptotic activity. We describe proliferative and apoptotic rates in different canine lymphoma subtypes at diagnosis. Flow cytometry (FC) was used to assess the percentage of proliferating cells (Ki67%) and of apoptotic cells (AnnV%) in 128 lymph node (LN) aspirates from dogs with lymphoma. Proliferation/apoptosis ratio (PAR) and turnover index (TI; Ki67% + AnnV%) were then calculated for each case. High-grade B-cell lymphomas showed high values for both Ki67% and AnnV%, low-grade B-cell lymphomas showed low Ki67% and high AnnV%, high-grade T-cell lymphomas showed high Ki67% and low AnnV%, and low-grade T-cell lymphomas showed low levels of both parameters. Lymphoblastic lymphomas had the highest PAR values. High-grade B-cell lymphomas had the highest TI values while small clear cells lymphomas the lowest. The panorama of proliferative and apoptotic activity widely varies among lymphoma subtypes. Our results lay the ground for future clinical and pharmacological studies.
Identifiants
pubmed: 33779558
pii: S0034-5288(20)31046-8
doi: 10.1016/j.rvsc.2020.10.008
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
324-328Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.