Sensitive electrochemical biosensor combined with isothermal amplification for point-of-care COVID-19 tests.
COVID-19
Coronavirus
Electrochemical detection method
Recombinase polymerase amplification
SARS-CoV-2
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 Jun 2021
15 Jun 2021
Historique:
received:
05
01
2021
revised:
08
03
2021
accepted:
12
03
2021
pubmed:
30
3
2021
medline:
27
4
2021
entrez:
29
3
2021
Statut:
ppublish
Résumé
We report an electrochemical biosensor combined with recombinase polymerase amplification (RPA) for rapid and sensitive detection of severe acute respiratory syndrome coronavirus 2. The electrochemical biosensor based on a multi-microelectrode array allows the detection of multiple target genes by differential pulse voltammetry. The RPA reaction involves hybridization of the RPA amplicon with thiol-modified primers immobilized on the working electrodes, which leads to a reduction of current density as amplicons accumulate. The assay results in shorter "sample-to-answer" times than conventional PCR without expensive thermo-cycling equipment. The limits of detection are about 0.972 fg/μL (RdRP gene) and 3.925 fg/μL (N gene), which are slightly lower than or comparable to that of RPA assay results obtained by gel electrophoresis without post-amplification purification. The combination of electrochemical biosensors and the RPA assay is a rapid, sensitive, and convenient platform that can be potentially used as a point-of-care test for the diagnosis of COVID-19.
Identifiants
pubmed: 33780853
pii: S0956-5663(21)00205-0
doi: 10.1016/j.bios.2021.113168
pmc: PMC7970423
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113168Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.
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