A haemagglutination test for rapid detection of antibodies to SARS-CoV-2.
Agglutination Tests
/ methods
Antibodies, Monoclonal
/ immunology
Antibodies, Viral
/ analysis
COVID-19
/ blood
COVID-19 Testing
/ methods
Enzyme-Linked Immunosorbent Assay
/ methods
Hemagglutination Tests
/ methods
Humans
Point-of-Care Systems
Polymerase Chain Reaction
SARS-CoV-2
/ immunology
Sensitivity and Specificity
Seroconversion
Spike Glycoprotein, Coronavirus
/ immunology
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 03 2021
29 03 2021
Historique:
received:
13
10
2020
accepted:
17
02
2021
entrez:
30
3
2021
pubmed:
31
3
2021
medline:
10
4
2021
Statut:
epublish
Résumé
Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.
Identifiants
pubmed: 33782398
doi: 10.1038/s41467-021-22045-y
pii: 10.1038/s41467-021-22045-y
pmc: PMC8007702
doi:
Substances chimiques
Antibodies, Monoclonal
0
Antibodies, Viral
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1951Subventions
Organisme : Department of Health
ID : 200927
Pays : United Kingdom
Organisme : Department of Health
ID : IS-BRC-1215-20013
Pays : United Kingdom
Organisme : NIAID NIH HHS
ID : HHSN272201400008C
Pays : United States
Organisme : Wellcome Trust
ID : 110110Z/15/Z
Pays : United Kingdom
Organisme : Cancer Research UK
ID : C18616/A25153
Pays : United Kingdom
Organisme : Department of Health
ID : 200907
Pays : United Kingdom
Organisme : Wellcome Trust
ID : WT204969/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 095541/A/11/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 109965/Z/15/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P021336/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L006588/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_19059
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 205228/Z/16/Z
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_19025
Pays : United Kingdom
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