Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids.
Cell Membrane
Cell culture
Cell isolation
Exomeres
Exosomes
Extracellular vesicles and particles
Isolation
Journal
STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501
Informations de publication
Date de publication:
19 03 2021
19 03 2021
Historique:
entrez:
31
3
2021
pubmed:
1
4
2021
medline:
1
4
2021
Statut:
epublish
Résumé
We developed a modified protocol, based on differential ultracentrifugation (dUC), to isolate extracellular vesicles and particles (specifically exomeres) (EVPs) from various human and murine sources, including cell lines, surgically resected tumors and adjacent tissues, and bodily fluids, such as blood, lymphatic fluid, and bile. The diversity of these samples requires robust and highly reproducible protocols and refined isolation technology, such as asymmetric-flow field-flow fractionation (AF4). Our isolation protocol allows for preparation of EVPs for various downstream applications, including proteomic profiling. For complete details on the use and execution of this protocol, please refer to Hoshino et al. (2020).
Identifiants
pubmed: 33786456
doi: 10.1016/j.xpro.2020.100225
pii: S2666-1667(20)30212-4
pmc: PMC7988237
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Pagination
100225Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA218513
Pays : United States
Organisme : NCATS NIH HHS
ID : UL1 TR002384
Pays : United States
Informations de copyright
© 2020 The Author(s).
Déclaration de conflit d'intérêts
D.L., A.H., H.S.K., and L.B. have filed a US patent application related to this work.
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