Structure of plant photosystem I-light harvesting complex I supercomplex at 2.4 Å resolution.
crystal structure, Lhca, LHCI, light-harvesting
pea
photosynthesis
photosystem I
Journal
Journal of integrative plant biology
ISSN: 1744-7909
Titre abrégé: J Integr Plant Biol
Pays: China (Republic : 1949- )
ID NLM: 101250502
Informations de publication
Date de publication:
Jul 2021
Jul 2021
Historique:
received:
10
12
2020
accepted:
14
03
2021
pubmed:
1
4
2021
medline:
28
12
2021
entrez:
31
3
2021
Statut:
ppublish
Résumé
Photosystem I (PSI) is one of the two photosystems in photosynthesis, and performs a series of electron transfer reactions leading to the reduction of ferredoxin. In higher plants, PSI is surrounded by four light-harvesting complex I (LHCI) subunits, which harvest and transfer energy efficiently to the PSI core. The crystal structure of PSI-LHCI supercomplex has been analyzed up to 2.6 Å resolution, providing much information on the arrangement of proteins and cofactors in this complicated supercomplex. Here we have optimized crystallization conditions, and analyzed the crystal structure of PSI-LHCI at 2.4 Å resolution. Our structure showed some shift of the LHCI, especially the Lhca4 subunit, away from the PSI core, suggesting the indirect connection and inefficiency of energy transfer from this Lhca subunit to the PSI core. We identified five new lipids in the structure, most of them are located in the gap region between the Lhca subunits and the PSI core. These lipid molecules may play important roles in binding of the Lhca subunits to the core, as well as in the assembly of the supercomplex. The present results thus provide novel information for the elucidation of the mechanisms for the light-energy harvesting, transfer and assembly of this supercomplex.
Substances chimiques
Photosystem I Protein Complex
0
Chlorophyll
1406-65-1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1367-1381Subventions
Organisme : National Key Research and Development Program of China
Organisme : Chinese Academy of Sciences Key Research Program of Frontier Sciences
Organisme : Strategic Priority Research Program of CAS
Organisme : National Basic Research Program of China
Organisme : National Natural Science Foundation of China
Organisme : Youth Innovation Promotion Association of CAS
Organisme : Taishan Scholars Project, and the Natural Science Foundation of Shandong Province China
Informations de copyright
© 2021 Institute of Botany, Chinese Academy of Sciences.
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