Cut-off values of serum IgG4 among three reagents, including a novel IgG4 reagent: a multicenter study.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
31 03 2021
Historique:
received: 16 08 2020
accepted: 04 03 2021
entrez: 1 4 2021
pubmed: 2 4 2021
medline: 21 10 2021
Statut: epublish

Résumé

Elevated serum IgG4 is a useful marker of IgG4-related disease (IgG4-RD) activity. However, there is no uniformity in the cut-off values of IgG4 among the various reagents. The aim of this study was to compare the measured and cut-off values of IgG4 assessed using three different reagents. This study enrolled 466 IgG4-RD and non-IgG4-RD patients who required measurement of serum IgG4 levels to diagnose or treat IgG4-RD. Serum IgG4 was measured using three reagents: N-assay LA IgG4 Nittobo (Nittobo), BS-NIA IgG4 (TBS), and N Latex IgG4 (Siemens). The values obtained using the three reagents were compared, and cut-off values were calculated for each. Although there was good correlation among the results with the three reagents, the measured and cut-off values were all different. The Nittobo values were 1.4 times the TBS values and the TBS values were almost half those of the Siemens values. ROC curve analysis showed cut-off values for the Nittobo, TBS, and Siemens reagents of 1.42, 1.31, and 2.38 g/L, respectively. The measured and cut-off values of serum IgG4 vary depending on the reagents used for the assay, although there is good correlation among the values measured by the three reagents.

Identifiants

pubmed: 33790306
doi: 10.1038/s41598-021-86024-5
pii: 10.1038/s41598-021-86024-5
pmc: PMC8012344
doi:

Substances chimiques

Immunoglobulin G 0
Reagent Kits, Diagnostic 0

Types de publication

Evaluation Study Journal Article Multicenter Study Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

7280

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Auteurs

Yoko Usami (Y)

Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.

Mitsutoshi Sugano (M)

Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.

Takeshi Uehara (T)

Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan. tuehara@shinshu-u.ac.jp.
Department of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan. tuehara@shinshu-u.ac.jp.

Masayoshi Koinuma (M)

Faculty of Pharmaceutical Sciences, Teikyo Heisei University, Tokyo, Japan.
Center of Clinical Research, Shinshu University Hospital, Matsumoto, Japan.

Nau Ishimine (N)

Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.

Kenji Kawasaki (K)

Department of Laboratory Medicine, Chiba University School of Medicine, Chiba, Japan.

Kazuyoshi Yamauchi (K)

Department of Laboratory Medicine, Faculty of Medicine, University of Tsukuba, Tsukuba, Japan.

Hideaki Hamano (H)

Nagano Prefectural Kiso Hospital, Kiso, Japan.

Takayuki Honda (T)

Department of Laboratory Medicine, Shinshu University Hospital, Matsumoto, Japan.
Department of Laboratory Medicine, Shinshu University School of Medicine, 3-1-1 Asahi, Matsumoto, 390-8621, Japan.

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