Comparison of platforms for testing antibodies to Chlamydia trachomatis antigens in the Democratic Republic of the Congo and Togo.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
31 03 2021
31 03 2021
Historique:
received:
20
11
2020
accepted:
08
03
2021
entrez:
1
4
2021
pubmed:
2
4
2021
medline:
21
10
2021
Statut:
epublish
Résumé
Trachoma, caused by repeated ocular infection with Chlamydia trachomatis (Ct), is targeted for elimination as a public health problem. Serological testing for antibodies is promising for surveillance; determining useful thresholds will require collection of serological data from settings with different prevalence of the indicator trachomatous inflammation-follicular (TF). Dried blood spots were collected during trachoma mapping in two districts each of Togo and Democratic Republic of the Congo. Anti-Ct antibodies were detected by multiplex bead assay (MBA) and three different lateral flow assays (LFA) and seroprevalence and seroconversion rate (SCR) were determined. By most tests, the district with > 5% TF (the elimination threshold) had five-sixfold higher seroprevalence and tenfold higher SCR than districts with < 5% TF. The agreement between LFA and MBA was improved using a black latex developing reagent. These data show optimization of antibody tests against Ct to better differentiate districts above or below trachoma elimination thresholds.
Identifiants
pubmed: 33790370
doi: 10.1038/s41598-021-86639-8
pii: 10.1038/s41598-021-86639-8
pmc: PMC8012353
doi:
Substances chimiques
Antibodies, Bacterial
0
Antigens, Bacterial
0
Types de publication
Clinical Trial
Comparative Study
Journal Article
Multicenter Study
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
7225Subventions
Organisme : World Health Organization
ID : 001
Pays : International
Commentaires et corrections
Type : ErratumIn
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