Detection and Labeling of Small Non-Coding RNAs by Splinted Ligation.
Bridge oligonucleotide
Ligation
Small RNA
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2021
2021
Historique:
entrez:
1
4
2021
pubmed:
2
4
2021
medline:
23
6
2021
Statut:
ppublish
Résumé
Discovery and characterization of microRNAs (miRNAs) and other families of small RNAs lead researchers to study their structures/functions and their expression patterns. The splinted ligation method described here is based on nucleic acid hybridization. It is optimized for the direct labeling and quantitative detection of small RNAs. A specific bridge DNA oligonucleotide is used, which is perfectly complementary to both the target small RNA and a labeled ligation nucleic acid. The target RNA is subsequently labeled by ligation, detected by analysis in denaturing conditions, and quantified by phosphorimaging. The protocol does not require any specific material, and the procedure is fast and sensitive.
Identifiants
pubmed: 33792872
doi: 10.1007/978-1-0716-1386-3_7
doi:
Substances chimiques
MicroRNAs
0
Oligonucleotide Probes
0
DNA Ligases
EC 6.5.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
65-72Références
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