Leptospirosis diagnosis among patients suspected of dengue fever in Brazil.
Leptospira
Microscopic agglutination test
PCR
Unreported disease
Zoonosis
Journal
The journal of venomous animals and toxins including tropical diseases
ISSN: 1678-9199
Titre abrégé: J Venom Anim Toxins Incl Trop Dis
Pays: Brazil
ID NLM: 101201501
Informations de publication
Date de publication:
26 Mar 2021
26 Mar 2021
Historique:
entrez:
2
4
2021
pubmed:
3
4
2021
medline:
3
4
2021
Statut:
epublish
Résumé
The early symptoms of leptospirosis and dengue fever are difficult to distinguish and can cause diagnostic confusion. Due to the large dengue epidemics that has occurred in Brazil in recent years, it is possible that cases of leptospirosis were unreported. Therefore, we performed a retrospective study to detect leptospirosis in patients who were tested for dengue, but whose laboratory diagnoses were negative. Sera samples from 2,017 patients from 48 cities located in the central region of São Paulo state, Brazil, were studied. All samples were subjected to the microscopic agglutination test (MAT), 305 of which were taken from patients five days or less since the onset of symptoms, and were additionally subjected to real-time polymerase chain reaction (PCR). The overall prevalence of leptospirosis cases was 21 (1.04%), with 20 through MAT (18 for Icterohaemorrhagiae and two for the Cynopteri serogroup) and one through PCR (amplicon sequencing compatible with The results indicated that patients suspected of dengue fever had evidence of leptospirosis or
Sections du résumé
BACKGROUND
BACKGROUND
The early symptoms of leptospirosis and dengue fever are difficult to distinguish and can cause diagnostic confusion. Due to the large dengue epidemics that has occurred in Brazil in recent years, it is possible that cases of leptospirosis were unreported. Therefore, we performed a retrospective study to detect leptospirosis in patients who were tested for dengue, but whose laboratory diagnoses were negative.
METHODS
METHODS
Sera samples from 2,017 patients from 48 cities located in the central region of São Paulo state, Brazil, were studied. All samples were subjected to the microscopic agglutination test (MAT), 305 of which were taken from patients five days or less since the onset of symptoms, and were additionally subjected to real-time polymerase chain reaction (PCR).
RESULTS
RESULTS
The overall prevalence of leptospirosis cases was 21 (1.04%), with 20 through MAT (18 for Icterohaemorrhagiae and two for the Cynopteri serogroup) and one through PCR (amplicon sequencing compatible with
CONCLUSION
CONCLUSIONS
The results indicated that patients suspected of dengue fever had evidence of leptospirosis or
Identifiants
pubmed: 33796136
doi: 10.1590/1678-9199-JVATITD-2020-0118
pmc: PMC7996315
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e20200118Déclaration de conflit d'intérêts
Competing interests: The authors declare no conflicts of interest.
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