Bone regeneration in both small and large preclinical bone defect models using an injectable polymer-based substitute containing hydroxyapatite and reconstituted with saline or autologous blood.
bone regeneration
injectable matrix
natural polysaccharides
oral surgery
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
revised:
18
03
2021
received:
28
09
2020
accepted:
22
03
2021
pubmed:
3
4
2021
medline:
8
3
2022
entrez:
2
4
2021
Statut:
ppublish
Résumé
Microbeads consisting of pullulan and dextran supplemented with hydroxyapatite have recently been developed for bone tissue engineering applications. Here, we evaluate the bone formation in two different preclinical models after injection of microbeads reconstituted with either saline buffer or autologous blood. Addition of saline solution or autologous blood to dried microbeads packaged into syringes allowed an easy injection. In the first rat bone defect model performed in the femoral condyle, microcomputed tomography performed after 30 and 60 days revealed an important mineralization process occurring around and within the core of the microbeads in both conditions. Bone volume/total volume measurements revealed no significant differences between the saline solution and the autologous blood groups. Histologically, osteoid tissue was evidenced around and in contact of the microbeads in both conditions. Using the sinus lift model performed in sheep, cone beam computed tomography revealed an important mineralization inside the sinus cavity for both groups after 3 months of implantation. Representative Masson trichrome staining images showed that bone formation occurs at the periphery and inside the microbeads in both conditions. Quantitative evaluation of the new bone formation displayed no significant differences between groups. In conclusion, reconstitution of microbeads with autologous blood did not enhance the regenerative capacity of these microbeads compared to the saline buffer group. This study is of particular interest for clinical applications in oral and maxillofacial surgery.
Substances chimiques
Polymers
0
Saline Solution
0
Durapatite
91D9GV0Z28
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1840-1848Informations de copyright
© 2021 Wiley Periodicals LLC.
Références
Elgali I, Omar O, Dahlin C, Thomsen P. Guided bone regeneration: materials and biological mechanisms revisited. Eur J Oral Sci. 2017;125:315-337.
Gilbert Triplett R, Budinskaya O. New frontiers in biomaterials. Oral Maxillofac Surg Clin North Am. 2017;29:105-115.
Barradas A, Yuan H, van Blitterswijk C, Habibovic P. Osteoinductive biomaterials: current knowledge of properties, experimental models and biological mechanisms. Eur Cell Mater. 2011;21:407-429.
Sammartino G, Ehrenfest DMD, Shibli JA, Galindo-Moreno P. Tissue engineering and dental implantology: biomaterials, new technologies, and stem cells. Biomed Res Int. 2016;2016:1-3.
Zhang Z. Injectable biomaterials for stem cell delivery and tissue regeneration. Expert Opin Biol Ther. 2017;17:49-62.
Ercan H, Durkut S, Koc-Demir A, Elçin AE, Elçin YM. Clinical applications of injectable biomaterials. In: Chun HJ, Park K, Kim C-H, Khang G, eds. Novel Biomaterials for Regenerative Medicine. Singapore: Springer Singapore; 2020.
No YJ, Roohani-Esfahani S, Zreiqat H. Nanomaterials: the next step in injectable bone cements. Nanomedicine. 2014;9:1745-1764.
Ogawa T, Kurita K, Imai T, Miyake M. Bone carrier technique with disposable syringe. Br J Oral Maxillofac Surg. 2017;55:e3-e4.
Santos IGBP, Santana CMM, Alves ATNN, Uzeda MJPG, Calasans-Maia MD, Santana RB. Effects of methods of hydration of a biphasic ceramic graft on bone regeneration of extraction socket defects. J Periodontol. 2019;90:425-432.
Horowitz RA, Mazor Z, Miller RJ, Krauser J, Prasad HS, Rohrer MD. Clinical evaluation alveolar ridge preservation with a beta-tricalcium phosphate socket graft. Compend Contin Educ Dent. 2009;30:588-590.
Friedmann A, Dard M, Kleber B-M, Bernimoulin J-P, Bosshardt DD. Ridge augmentation and maxillary sinus grafting with a biphasic calcium phosphate: histologic and histomorphometric observations. Clin Oral Implants Res. 2009;20:708-714.
Barbeck M, Najman S, Stojanović S, et al. Addition of blood to a phycogenic bone substitute leads to increased in vivo vascularization. Biomed Mater. 2015;10:055007.
Boos A, Weigand A, Deschler G, et al. Autologous serum improves bone formation in a primary stable silica-embedded nanohydroxyapatite bone substitute in combination with mesenchymal stem cells and rhBMP-2 in the sheep model. IJN. 2014;19(9):5317-5339.
Fricain JC, Schlaubitz S, Le Visage C, et al. A nano-hydroxyapatite-Pullulan/dextran polysaccharide composite macroporous material for bone tissue engineering. Biomaterials. 2013;34:2947-2959.
Schlaubitz S, Derkaoui SM, Marosa L, Miraux S, Renard M, Catros S, Le Visage C, Letourneur D, Amédée J, Fricain J-C. Pullulan/dextran/nHA macroporous composite beads for bone repair in a femoral condyle defect in rats. Heymann D PLoS ONE 2014;9:e110251.
Ribot EJ, Tournier C, Aid-Launais R, et al. 3D anatomical and perfusion MRI for longitudinal evaluation of biomaterials for bone regeneration of femoral bone defect in rats. Sci Rep. 2017;7:6100.
Fricain JC, Aid R, Lanouar S, et al. In-vitro and in-vivo design and validation of an injectable polysaccharide-hydroxyapatite composite material for sinus floor augmentation. Dent Mater. 2018;34:1024-1035.
Catros S, Guillemot F, Lebraud E, et al. Physico-chemical and biological properties of a nano-hydroxyapatite powder synthesized at room temperature. IRBM. 2010;31:226-233.
Autissier A, Visage CL, Pouzet C, Chaubet F, Letourneur D. Fabrication of porous polysaccharide-based scaffolds using a combined freeze-drying/cross-linking process. Acta Biomater. 2010;6:3640-3648.
Guerrero J, Oliveira H, Catros S, et al. The use of Total human bone marrow fraction in a direct three-dimensional expansion approach for bone tissue engineering applications: focus on angiogenesis and Osteogenesis. Tissue Eng Part A. 2015;21:861-874.
Le Visage C, Derkaoui SM, Letourneur D. Crosslinked polysaccharide beads and their biomedical uses. WO/2012/028623, PCT/EP2011/064927.
Saffarzadeh A, Gauthier O, Bilban M, Bagot D'Arc M, Daculsi G. Comparison of two bone substitute biomaterials consisting of a mixture of fibrin sealant (Tisseel ® ) and MBCP ™ (TricOs ® ) with an autograft in sinus lift surgery in sheep. Clin Oral Implants Res. 2009;20:1133-1139.
Gutwald R, Haberstroh J, Stricker A, et al. Influence of rhBMP-2 on bone formation and osseointegration in different implant systems after sinus-floor elevation. An in vivo study on sheep. J Craniomaxillofac Surg. 2010;38:571-579.
Mastrangelo F, Quaresima R, Sebastianelli I, et al. Poly D,L-Lactide-co-glycolic acid grafting material in sinus lift. J Craniofac Surg. 2019;30:1073-1077.
Miron RJ, Fujioka-Kobayashi M, Hernandez M, et al. Injectable platelet rich fibrin (i-PRF): opportunities in regenerative dentistry? Clin Oral Invest. 2017;21:2619-2627.
Thorwarth M, Wehrhan F, Schultze-Mosgau S, Wiltfang J, Schlegel KA. PRP modulates expression of bone matrix proteins in vivo without long-term effects on bone formation. Bone. 2006;38:30-40.
Plachokova AS, Nikolidakis D, Mulder J, Jansen JA, Creugers NHJ. Effect of platelet-rich plasma on bone regeneration in dentistry: a systematic review. Clin Oral Implants Res. 2008;19:539-545.
Wiltfang J, Kloss FR, Kessler P, et al. Effects of platelet-rich plasma on bone healing in combination with autogenous bone and bone substitutes in critical-size defects. Clin Oral Implants Res. 2004;15:187-193.
Klongnoi B, Rupprecht S, Kessler P, et al. Lack of beneficial effects of platelet-rich plasma on sinus augmentation using a fluorohydroxyapatite or autogenous bone: an explorative study. J Clin Periodontol. 2006;33:500-509.
van Bergen CJA, Kerkhoffs GMMJ, Özdemir M, et al. Demineralized bone matrix and platelet-rich plasma do not improve healing of osteochondral defects of the talus: an experimental goat study. Osteoarthr Cartil. 2013;21:1746-1754.
Plachokova AS, van den Dolder J, Stoelinga PJ, Jansen JA. The bone regenerative effect of platelet-rich plasma in combination with an osteoconductive material in rat cranial defects. Clin Oral Implants Res. 2006;17:305-311.