Magnetic Nanoparticle-Based Hyperthermia Mediates Drug Delivery and Impairs the Tumorigenic Capacity of Quiescent Colorectal Cancer Stem Cells.


Journal

ACS applied materials & interfaces
ISSN: 1944-8252
Titre abrégé: ACS Appl Mater Interfaces
Pays: United States
ID NLM: 101504991

Informations de publication

Date de publication:
14 Apr 2021
Historique:
pubmed: 3 4 2021
medline: 11 8 2021
entrez: 2 4 2021
Statut: ppublish

Résumé

Cancer stem cells (CSCs) are the tumor cell subpopulation responsible for resistance to chemotherapy, tumor recurrence, and metastasis. An efficient therapy must act on low proliferating quiescent-CSCs (q-CSCs). We here investigate the effect of magnetic hyperthermia (MHT) in combination with local chemotherapy as a dual therapy to inhibit patient-derived colorectal qCR-CSCs. We apply iron oxide nanocubes as MHT heat mediators, coated with a thermoresponsive polymer (TR-Cubes) and loaded with DOXO (TR-DOXO) as a chemotherapeutic agent. The thermoresponsive polymer releases DOXO only at a temperature above 44 °C. In colony-forming assays, the cells exposed to TR-Cubes with MHT reveal that qCR-CSCs struggle to survive the heat damage and, with a due delay, restart the division of dormant cells. The eradication of qCR-CSCs with a complete stop of the colony formation was achieved only with TR-DOXO when exposed to MHT. The

Identifiants

pubmed: 33797220
doi: 10.1021/acsami.0c21349
pmc: PMC8045020
doi:

Substances chimiques

Antibiotics, Antineoplastic 0
Magnetite Nanoparticles 0
Doxorubicin 80168379AG

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

15959-15972

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Auteurs

Soraia Fernandes (S)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

Tamara Fernandez (T)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

Sabrina Metze (S)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

Preethi B Balakrishnan (PB)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

Binh T Mai (BT)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

John Conteh (J)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

Claudia De Mei (C)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

Alice Turdo (A)

PROMISE Department,Piazza delle Cliniche 2, University of Palermo, 90133 Palermo, Italy.

Simone Di Franco (S)

DICHIRONS Department, University of Palermo, Via del Vespro 129, 90133 Palermo, Italy.

Giorgio Stassi (G)

DICHIRONS Department, University of Palermo, Via del Vespro 129, 90133 Palermo, Italy.

Matilde Todaro (M)

PROMISE Department,Piazza delle Cliniche 2, University of Palermo, 90133 Palermo, Italy.

Teresa Pellegrino (T)

Istituto Italiano di Tecnologia (IIT), via Morego 30, 16163 Genova, Italy.

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Classifications MeSH