Kinetics of glucosinolate hydrolysis by myrosinase in Brassicaceae tissues: A high-performance liquid chromatography approach.

Brassicaceae / enzymology Chromatography, High Pressure Liquid Glucosinolates / chemistry Glycoside Hydrolases / metabolism Humans Hydrolysis Kinetics
Brassica oleracea L. var. botrytis Brassica oleracea L. var. italic Glucosinolate HPLC-based assay Kinetic study Myrosinase Spectrophotometric assay Substrate inhibition

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
01 Sep 2021
Historique:
received: 09 09 2020
revised: 09 03 2021
accepted: 13 03 2021
pubmed: 3 4 2021
medline: 14 5 2021
entrez: 2 4 2021
Statut: ppublish

Résumé

Glucosinolates are a group of secondary metabolites occurring in all the vegetables belonging to the Brassicaceae family. Upon tissue damage, glucosinolates are hydrolyzed by myrosinase to a series of degradation products, including isothiocyanates, which are important for their health-promoting effects in humans. The glucosinolate-myrosinase system has been characterized in several Brassica species, of which white mustard (Sinapis alba) has been studied the most. In this study, a new HPLC-UV assay to evaluate the activities and kinetics of myrosinases in aqueous extracts, which closely represent the physiological conditions of plant tissues, was developed. This method was tested on myrosinases extracted from broccoli and cauliflower inflorescences, employing sinigrin and glucoraphanin as substrates. The results showed a strong inhibition of both enzymes at high substrate concentrations. The main issues related to kinetic analysis on the glucosinolate-myrosinase system were also elucidated.

Identifiants

DOI: 10.1016/j.foodchem.2021.129634 PMID: 33799240
pubmed: 33799240
pii: S0308-8146(21)00640-3
doi: 10.1016/j.foodchem.2021.129634
pii:
doi:

Substances chimiques

Glucosinolates 0
Glycoside Hydrolases EC 3.2.1.-
thioglucosidase EC 3.2.1.147

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

129634

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Alessio Pardini (A)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy; Center for Colloid and Surface Science (CSGI), University of Florence, via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy. Electronic address: alessio.pardini@unisi.it.

Gabriella Tamasi (G)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy; Center for Colloid and Surface Science (CSGI), University of Florence, via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy. Electronic address: gabriella.tamasi@unisi.it.

Federica De Rocco (F)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy.

Claudia Bonechi (C)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy; Center for Colloid and Surface Science (CSGI), University of Florence, via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy.

Marco Consumi (M)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy; National Interuniversity Consortium of Materials Science and Technology (INSTM), via Giuseppe Giusti 9, 50121 Florence, Italy.

Gemma Leone (G)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy; National Interuniversity Consortium of Materials Science and Technology (INSTM), via Giuseppe Giusti 9, 50121 Florence, Italy.

Agnese Magnani (A)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy; National Interuniversity Consortium of Materials Science and Technology (INSTM), via Giuseppe Giusti 9, 50121 Florence, Italy.

Claudio Rossi (C)

Department of Biotechnology, Chemistry and Pharmacy (DBCF), University of Siena, via Aldo Moro 2, 53100 Siena, Italy; Center for Colloid and Surface Science (CSGI), University of Florence, via della Lastruccia 3, 50019 Sesto Fiorentino (FI), Italy.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Kinetics of glucosinolate hydrolysis by...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Kinetics of glucosinolate hydrolysis by...
questionsmedicales.fr › Glucides › Hétérosides › Thioglycosides › Thioglucosides › Glucosinolates › Kinetics of glucosinolate hydrolysis by...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Kinetics of glucosinolate hydrolysis by...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Kinetics of glucosinolate hydrolysis by...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › Kinetics of glucosinolate hydrolysis by...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Kinetics of glucosinolate hydrolysis by...