Different miRNA Profiles in Plasma Derived Small and Large Extracellular Vesicles from Patients with Neurodegenerative Diseases.
Alzheimer’s disease
Parkinson’s disease
RNA-seq
amyotrophic lateral sclerosis
frontotemporal dementia
large extracellular vesicles
miRNAs
small extracellular vesicles
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
08 Mar 2021
08 Mar 2021
Historique:
received:
05
02
2021
revised:
01
03
2021
accepted:
02
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
23
4
2021
Statut:
epublish
Résumé
Identifying biomarkers is essential for early diagnosis of neurodegenerative diseases (NDs). Large (LEVs) and small extracellular vesicles (SEVs) are extracellular vesicles (EVs) of different sizes and biological functions transported in blood and they may be valid biomarkers for NDs. The aim of our study was to investigate common and different miRNA signatures in plasma derived LEVs and SEVs of Alzheimer's disease (AD), Parkinson's disease (PD), Amyotrophic Lateral Sclerosis (ALS) and Fronto-Temporal Dementia (FTD) patients. LEVs and SEVs were isolated from plasma of patients and healthy volunteers (CTR) by filtration and differential centrifugation and RNA was extracted. Small RNAs libraries were carried out by Next Generation Sequencing (NGS). MiRNAs discriminate all NDs diseases from CTRs and they can provide a signature for each NDs. Common enriched pathways for SEVs were instead linked to ubiquitin mediated proteolysis and Toll-like receptor signaling pathways and for LEVs to neurotrophin signaling and Glycosphingolipid biosynthesis pathway. LEVs and SEVs are involved in different pathways and this might give a specificity to their role in the spreading of the disease. The study of common and different miRNAs transported by LEVs and SEVs can be of great interest for biomarker discovery and for pathogenesis studies in neurodegeneration.
Identifiants
pubmed: 33800495
pii: ijms22052737
doi: 10.3390/ijms22052737
pmc: PMC7962970
pii:
doi:
Substances chimiques
Circulating MicroRNA
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministero della Salute
ID : Young research project GR-2016-02361552
Organisme : Ministero della Salute
ID : Ricerca Corrente 2018-2020
Organisme : Ministero della Salute
ID : 5*1000
Organisme : Fondazione Cariplo
ID : Extracellular vesicles in the pathogenesis of Frontotemporal Dementia 2017-0747
Organisme : Fondazione Cariplo
ID : Association between frailty trajectories and biological markers of aging 2017-0557
Organisme : Fondazione Regionale per la Ricerca Biomedica
ID : FRRB 2015-0023
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