Spatial and Temporal Virus Load Dynamics of SARS-CoV-2: A Single-Center Cohort Study.
COVID-19
RT-PCR
SARS-CoV-2
molecular diagnostics
viral shedding
Journal
Diagnostics (Basel, Switzerland)
ISSN: 2075-4418
Titre abrégé: Diagnostics (Basel)
Pays: Switzerland
ID NLM: 101658402
Informations de publication
Date de publication:
03 03 2021
03 03 2021
Historique:
received:
25
01
2021
revised:
22
02
2021
accepted:
22
02
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
4
4
2021
Statut:
epublish
Résumé
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused an ongoing pandemic. Reverse transcription polymerase chain reaction (RT-PCR) is the gold standard for the detection of SARS-CoV-2 and has been applied to different specimen types. Understanding the virus load and virus detection frequency in different specimen types is important to improve diagnosis and estimate the duration of potential infectivity. We conducted a retrospective single-center cohort study on hospitalized and outpatients with SARS-CoV-2 infection. We analyzed the frequency of virus detection, virus load, and duration of the virus excretion in upper and lower respiratory specimens as well as stool and plasma. We found that the frequency of SARS-CoV-2 detection, the virus load, and duration of virus excretion was higher in lower respiratory tract (LRT) than in upper respiratory tract (URT) specimens. The duration of virus excretion was longer in patients requiring intensive care unit (ICU) admission. In conclusion, LRT specimens are the most appropriate specimen type for the detection and follow-up of SARS-CoV-2 infection. Duration of virus excretion is longer in severe cases of SARS-CoV-2 infection.
Identifiants
pubmed: 33802451
pii: diagnostics11030427
doi: 10.3390/diagnostics11030427
pmc: PMC7999170
pii:
doi:
Types de publication
Journal Article
Langues
eng
Investigateurs
Marc Lambert
Cécile Yelnik
Ryad Pokeerbux
Loïc Andre
Edgar Bakhache
Arnaud Scherpereel
Jacques Desbordes
Nathalie Bautin
Stéphanie Fry
Louise Duthoit
Nicolas Duhamel
Pascal De Groote
François Pusieux
Cédric Gaxatte
Jean-Baptiste Beuscart
Anne Charpentier
(A)
Patrick Golstein
(P)
Alain Facon
(A)
Francois Vanheems
(F)
Jerome Cuny
(J)
Roch Joly
(R)
Eric Wiel
(E)
Leslie Charbonnier-Dinner
(L)
Vincent Pegorado
(V)
Eric Kipnis
(E)
Marielle Boyer-Besseyre
(M)
Marie Boulo
(M)
Anne Bignon
(A)
Perrine Bortolotti
(P)
Marion Fajardy
(M)
Gilles Lebuffe
(G)
Remi Fackeure
(R)
Laurent Carpentier
(L)
Gregoire Andrieu
(G)
Benoit Capron
(B)
Jerome Onimus
(J)
Virginie Sanders
(V)
Antoine Jeanneteau
(A)
Djihad Amroun
(D)
Pauline Boddaert
(P)
Morgan Caplan
(M)
Nicolas Cousin
(N)
Thibault Duburcq
(T)
Arthur Durand
(A)
Ahmed El Kalioubie
(A)
Raphael Favory
(R)
Patrick Girardie
(P)
Julien Goutay
(J)
Marion Houard
(M)
Emmanuelle Jailliette
(E)
Mercedes Jourdain
(M)
Goeffrey Ledoux
(G)
Daniel Mathieu
(D)
Anne-Sophie Moreau
(AS)
Olivier Nigeon
(O)
Christopher Niles
(C)
Saad Nseir
(S)
Thierry Onimus
(T)
Erika Parmentier
(E)
Julien Poissy
(J)
Sébastien Preau
(S)
Laurent Robriquet
(L)
Anahita Rouze
(A)
Arthur Simmonet
(A)
Sophie Six
(S)
Aurélia Toussaint
(A)
Delphine Garrigue
(D)
Florence Lallemant
(F)
Aurelien Rohn
(A)
Lois Henry
(L)
Benjamin Bijok
(B)
Guillaume Strecker
(G)
Benoit Tavernier
(B)
Elsa Josefowicz
(E)
Pauline Devauchelle
(P)
Emmanuel Robin
(E)
Christophe Decoene
(C)
Slimane Ait Ouarab
(S)
Mouhammed Moussa
(M)
Olivier Joulin
(O)
Guillaume Leroy
(G)
Guillaume Gantois
(G)
Caroline Brandt
(C)
Noredine Saddouk
(N)
Celine Dupre
(C)
Xavier Leroy
(X)
Raphael Marie
(R)
Vincent Liu
(V)
Valentin Foulon
(V)
Sylvain Massias
(S)
Nicolas Rytter
(N)
Delphine Deblauwe
(D)
Vincent Pettigand
(V)
Christophe Muller
(C)
Jacques Desbordes
(J)
Catherine Dusson
(C)
Thierry Brousseau
(T)
Sophie Susen
(S)
Antoine Rauch
(A)
Fanny Lassalle
(F)
Annabelle Dupont
(A)
Delphine Corseaux
(D)
Emmanuelle Jeanpierre
(E)
Anne Bauters
(A)
Nathalie Trillot
(N)
Myriam Labalette
(M)
Guillaume Lefevre
(G)
Julie Demaret
(J)
Pauline Varlet
(P)
Mohamed Bou Saleh
(M)
Laurence Bocket
(L)
Enagnon Kazali Alidjinou
(EK)
Brigitte Prevost
(B)
Mouna Lazrek
(M)
Claire Tinez
(C)
Laurine Milliere
(L)
Ilka Engelmann
(I)
Youssef Bouarouro
(Y)
Didier Hober
(D)
Boualem Sendid
(B)
Olivier Nibourel
(O)
Antoine Tournoy
(A)
Marie-Christine Copin
(MC)
Romain Dubois
(R)
Jean-Baptiste Gibier
(JB)
Viviane Gnemmi
(V)
Sarah Humez
(S)
Malik Kadri
(M)
Emmanuelle Leteurtre
(E)
Romain Perbet
(R)
Dominique Deplanque
(D)
Aurélie Dozier
(A)
Ady Assaf
(A)
Marie-Charlotte Chopin
(MC)
Emmanuel Faure
(E)
Fanny Vuotto
(F)
Sophie Panaget
(S)
Karine Faure
(K)
Clara Leroy
(C)
Julie Prasivoravong
(J)
Céline Berthon
(C)
Laure Goursaud
(L)
François Provot
(F)
Hélène Lenci
(H)
Jean-François Bervar
(JF)
Anne Prevotat
(A)
Références
Clin Infect Dis. 2020 Sep 23;:
pubmed: 32965474
EBioMedicine. 2020 Aug;58:102916
pubmed: 32711256
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
JAMA Netw Open. 2020 Jun 1;3(6):e2011335
pubmed: 32525549
Lancet. 2020 Mar 28;395(10229):1054-1062
pubmed: 32171076
Clin Infect Dis. 2020 Jul 28;:
pubmed: 32720678
J Med Virol. 2021 Feb;93(2):719-725
pubmed: 32706393
N Engl J Med. 2020 Mar 19;382(12):1177-1179
pubmed: 32074444
Nat Microbiol. 2020 Apr;5(4):536-544
pubmed: 32123347
J Infect. 2020 Sep;81(3):357-371
pubmed: 32615199
Nat Med. 2020 May;26(5):672-675
pubmed: 32296168
Infect Dis Health. 2020 Aug;25(3):210-215
pubmed: 32473952
Wellcome Open Res. 2020 Jul 29;5:181
pubmed: 33283055
Nature. 2020 May;581(7809):465-469
pubmed: 32235945
J Infect Public Health. 2020 Jul;13(7):901-905
pubmed: 32534946
Lancet Infect Dis. 2020 May;20(5):565-574
pubmed: 32213337
Lancet Infect Dis. 2020 Jun;20(6):697-706
pubmed: 32224310
Eur J Clin Microbiol Infect Dis. 2020 Jun;39(6):1059-1061
pubmed: 32342252
J Infect. 2020 Aug;81(2):318-356
pubmed: 32283147
BMJ. 2020 Apr 21;369:m1443
pubmed: 32317267
J Infect. 2020 Aug;81(2):e31-e38
pubmed: 32535156