Noninvasive Tracking of mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cells after Subcutaneous Transplantation in Mice.
MIN6 cells
bioluminescence imaging
magnetic resonance imaging
subcutaneous transplantation
thermosensitive hydrogels
Journal
Polymers
ISSN: 2073-4360
Titre abrégé: Polymers (Basel)
Pays: Switzerland
ID NLM: 101545357
Informations de publication
Date de publication:
13 Mar 2021
13 Mar 2021
Historique:
received:
18
02
2021
revised:
11
03
2021
accepted:
11
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
4
4
2021
Statut:
epublish
Résumé
Recently, we demonstrated the feasibility of subcutaneous transplantation of MIN6 cells embedded in a scaffold with poly(ethylene glycol) methyl ether (mPEG)-poly(Ala) hydrogels. In this study, we further tracked these grafts using magnetic resonance (MR) and bioluminescence imaging. After being incubated overnight with chitosan-coated superparamagnetic iron oxide (CSPIO) nanoparticles and then mixed with mPEG-poly(Ala) hydrogels, MIN6 cells appeared as dark spots on MR scans. For in vivo experiments, we transfected MIN6 cells with luciferase and/or incubated them overnight with CSPIO overnight; 5 × 10
Identifiants
pubmed: 33805723
pii: polym13060885
doi: 10.3390/polym13060885
pmc: PMC7998640
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Chang Gung Memorial Hospital
ID : CMRPG3F0711-3 and CMRPG3G1961
Organisme : Chang Gung Memorial Hospital-National Tsing Hua University Joint Research Program 2019
ID : CORPG3I0031 and 108Q2505E1
Organisme : Chang Gung Memorial Hospital and National Tsing Hua University Joint Research Program CGMH-NTHU 2020
ID : CORPG3K0031 and 109Q2513E1
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