In Vitro Effects of Low Doses of β-Caryophyllene, Ascorbic Acid and d-Glucosamine on Human Chondrocyte Viability and Inflammation.
ascorbic acid
chondrocyte
d-glucosamine
inflammation
osteoarthritis
reactive oxygen species
β-caryophyllene
Journal
Pharmaceuticals (Basel, Switzerland)
ISSN: 1424-8247
Titre abrégé: Pharmaceuticals (Basel)
Pays: Switzerland
ID NLM: 101238453
Informations de publication
Date de publication:
23 Mar 2021
23 Mar 2021
Historique:
received:
08
02
2021
revised:
13
03
2021
accepted:
20
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
4
4
2021
Statut:
epublish
Résumé
β-caryophyllene (BCP), a plant-derived sesquiterpene, has been reported to have anti-inflammatory and antioxidant effects. The purpose of this study is to evaluate the effects of BCP in combination with ascorbic acid (AA) and d-glucosamine (GlcN) against macrophage-mediated inflammation on in vitro primary human chondrocytes. Changes in cell viability, intracellular ROS generation, gene expression of pro-inflammatory mediators, metalloproteinases (MMPs), collagen type II and aggrecan were analyzed in primary human chondrocytes exposed to the conditioned medium (CM) of activated U937 monocytes and subsequently treated with BCP alone or in combination with AA and GlcN. The CM-induced chondrocyte cytotoxicity was reduced by the presence of low doses of BCP alone or in combination with AA and GlcN. The exposure of cells to CM significantly increased
Identifiants
pubmed: 33806983
pii: ph14030286
doi: 10.3390/ph14030286
pmc: PMC8005039
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : UNI-Impresa, University of Padova, Italy
ID : 0
Références
Osteoarthritis Cartilage. 2016 Sep;24(9):1613-21
pubmed: 27084348
Bioorg Med Chem Lett. 2014 Jul 15;24(14):3168-74
pubmed: 24856059
Biomed Pharmacother. 2018 Jul;103:1086-1091
pubmed: 29710667
Drugs Aging. 2012 Sep;29(9):717-31
pubmed: 23018608
Arthritis Res Ther. 2008;10(2):R43
pubmed: 18416822
Mol Med Rep. 2016 Jun;13(6):4569-76
pubmed: 27082728
Cannabis Cannabinoid Res. 2016 Jan 01;1(1):3-15
pubmed: 28861474
Biomolecules. 2019 Jul 31;9(8):
pubmed: 31370242
Histochem Cell Biol. 2006 Aug;126(2):177-87
pubmed: 16395612
Mol Med Rep. 2015 Nov;12(5):7086-92
pubmed: 26300283
J Biomed Mater Res. 1999 Sep 5;46(3):337-46
pubmed: 10397990
Biomed Res Int. 2013;2013:284873
pubmed: 24069595
Biochem Pharmacol. 2019 Jan;159:154-171
pubmed: 30529211
Int J Mol Sci. 2016 Dec 27;18(1):
pubmed: 28035982
Lancet. 2015 Jul 25;386(9991):376-87
pubmed: 25748615
Biomedicines. 2020 Jun 17;8(6):
pubmed: 32560286
Neuroscience. 2014 Oct 24;279:220-31
pubmed: 25194788
Fibrogenesis Tissue Repair. 2009 Dec 15;2:7
pubmed: 20003476
Cells. 2020 May 26;9(6):
pubmed: 32466461
Biochem Pharmacol. 2007 May 1;73(9):1434-45
pubmed: 17291458
J Biomed Mater Res B Appl Biomater. 2012 Nov;100(8):2073-81
pubmed: 22865740
Oncogene. 1999 Nov 22;18(49):6853-66
pubmed: 10602461
Osteoarthritis Cartilage. 2011 Jan;19(1):111-7
pubmed: 21035559
RMD Open. 2015 Aug 15;1(Suppl 1):e000061
pubmed: 26557379
Biochem J. 1998 Oct 1;335 ( Pt 1):59-66
pubmed: 9742213
Can Fam Physician. 2004 Feb;50:241-8
pubmed: 15000335
Annu Rev Pharmacol Toxicol. 2018 Jan 6;58:583-601
pubmed: 28992429
Int J Toxicol. 2016 Sep;35(5):558-67
pubmed: 27358239
Curr Pharm Des. 2016;22(21):3237-64
pubmed: 26965491
Cell Mol Life Sci. 2016 Dec;73(23):4449-4470
pubmed: 27402121
Int J Clin Pract. 2012 Jul;66(7):699-704
pubmed: 22698422
Hepatology. 2017 Jan;65(1):363-373
pubmed: 27677775
Adv Ther. 2019 Nov;36(11):3221-3237
pubmed: 31494830
Arthritis Res Ther. 2014 Sep 27;16(5):437
pubmed: 25260980
Eur Neuropsychopharmacol. 2014 Apr;24(4):608-20
pubmed: 24210682
Biomed Pharmacother. 2019 Feb;110:145-154
pubmed: 30469079
J Immunol Methods. 1986 May 22;89(2):271-7
pubmed: 3486233
Am J Pathol. 2011 Mar;178(3):1153-66
pubmed: 21356367
Nucleic Acids Res. 2001 Nov 1;29(21):4361-72
pubmed: 11691923
PLoS One. 2013 Nov 25;8(11):e80440
pubmed: 24282543
Arthritis Res Ther. 2006;8(6):R173
pubmed: 17109745
Arthritis Rheum. 2012 Jun;64(6):1697-707
pubmed: 22392533
Br J Pharmacol. 2016 Jun;173(12):1899-910
pubmed: 27077495
Rheumatol Int. 2006 Aug;26(10):900-3
pubmed: 16468044