Silk Fiber-Reinforced Hyaluronic Acid-Based Hydrogel for Cartilage Tissue Engineering.
Aged
Biocompatible Materials
/ metabolism
Cartilage
/ cytology
Cartilage, Articular
/ cytology
Cell Survival
/ physiology
Cells, Cultured
Chondrocytes
/ metabolism
Chondrogenesis
Fibroins
/ metabolism
Humans
Hyaluronic Acid
/ pharmacology
Hydrogels
/ metabolism
Middle Aged
Porosity
Silk
/ metabolism
Tissue Engineering
/ methods
Tissue Scaffolds
/ chemistry
TGF-β1
autologous chondrocyte implantation
biomaterials
cartilage
chondrocytes
hyaluronic acid
silk fibroin
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
31 Mar 2021
31 Mar 2021
Historique:
received:
27
02
2021
revised:
21
03
2021
accepted:
25
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
11
5
2021
Statut:
epublish
Résumé
A continuing challenge in cartilage tissue engineering for cartilage regeneration is the creation of a suitable synthetic microenvironment for chondrocytes and tissue regeneration. The aim of this study was to develop a highly tunable hybrid scaffold based on a silk fibroin matrix (SM) and a hyaluronic acid (HA) hydrogel. Human articular chondrocytes were embedded in a porous 3-dimensional SM, before infiltration with tyramine modified HA hydrogel. Scaffolds were cultured in chondropermissive medium with and without TGF-β1. Cell viability and cell distribution were assessed using CellTiter-Blue assay and Live/Dead staining. Chondrogenic marker expression was detected using qPCR. Biosynthesis of matrix compounds was analyzed by dimethylmethylene blue assay and immuno-histology. Differences in biomaterial stiffness and stress relaxation were characterized using a one-step unconfined compression test. Cell morphology was investigated by scanning electron microscopy. Hybrid scaffold revealed superior chondro-inductive and biomechanical properties compared to sole SM. The presence of HA and TGF-β1 increased chondrogenic marker gene expression and matrix deposition. Hybrid scaffolds offer cytocompatible and highly tunable properties as cell-carrier systems, as well as favorable biomechanical properties.
Identifiants
pubmed: 33807323
pii: ijms22073635
doi: 10.3390/ijms22073635
pmc: PMC8036422
pii:
doi:
Substances chimiques
Biocompatible Materials
0
Hydrogels
0
Silk
0
Hyaluronic Acid
9004-61-9
Fibroins
9007-76-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Christian-Albrechts-Universität zu Kiel
ID : F359958
Références
Acta Biomater. 2020 Jan 1;101:293-303
pubmed: 31726249
Acta Biomater. 2019 Jan 1;83:233-244
pubmed: 30366137
Adv Exp Med Biol. 2018;1077:371-387
pubmed: 30357699
J Tissue Eng. 2018 Oct 08;9:2041731418802438
pubmed: 30305887
Cell. 1978 Dec;15(4):1313-21
pubmed: 729001
Mater Sci Eng C Mater Biol Appl. 2021 Jan;120:111701
pubmed: 33545860
Injury. 2008 Apr;39 Suppl 1:S77-87
pubmed: 18313475
Mater Sci Eng C Mater Biol Appl. 2017 Jul 1;76:810-817
pubmed: 28482595
FEBS Lett. 2002 Sep 25;528(1-3):101-8
pubmed: 12297287
Carbohydr Polym. 2015 Jan 22;115:325-33
pubmed: 25439901
Biomaterials. 2010 Feb;31(4):631-40
pubmed: 19819543
J Funct Biomater. 2018 Jun 24;9(3):
pubmed: 29937524
Tissue Eng. 2004 Jul-Aug;10(7-8):1277-86
pubmed: 15363182
Knee. 2016 Jun;23(3):426-35
pubmed: 26947215
Appl Biochem Biotechnol. 2017 Aug;182(4):1548-1563
pubmed: 28138929
N Engl J Med. 1994 Oct 6;331(14):889-95
pubmed: 8078550
Biomaterials. 2011 Feb;32(4):1032-40
pubmed: 21074264
Cartilage. 2013 Jan;4(1):12-9
pubmed: 26069648
Cell. 1982 Aug;30(1):215-24
pubmed: 7127471
Am J Sports Med. 2020 Jan;48(1):210-221
pubmed: 31877102
Arthritis Rheum. 2005 Jul;52(7):2033-9
pubmed: 15986359
Biomaterials. 2016 Aug;98:1-22
pubmed: 27177218
Polymers (Basel). 2017 Dec 04;9(12):
pubmed: 30965974
J Inflamm (Lond). 2016 Sep 13;13(1):31
pubmed: 27625590
Tissue Eng Part A. 2017 Aug;23(15-16):859-872
pubmed: 28330431
Biomaterials. 2006 Sep;27(25):4434-42
pubmed: 16677707
Cartilage. 2010 Jan;1(1):29-42
pubmed: 26069534
Adv Funct Mater. 2014 Aug 6;24(29):4615-4624
pubmed: 25395921
Orthop J Sports Med. 2019 Jul 10;7(7):2325967119854442
pubmed: 31317047
Arch Biochem Biophys. 2000 Sep 15;381(2):205-12
pubmed: 11032407
Tissue Eng Part A. 2018 Oct;24(19-20):1481-1491
pubmed: 29681215
Nat Mater. 2017 Dec;16(12):1243-1251
pubmed: 28967913
Osteoarthritis Cartilage. 2007 May;15(5):479-86
pubmed: 17291789
Cartilage. 2012 Jul;3(3):205-21
pubmed: 26069634