NMR Experiments Shed New Light on Glycan Recognition by Human and Murine Norovirus Capsid Proteins.
chemical shift perturbation
histo blood group antigens
norovirus P-domain
protein-carbohydrate recognition
sialoglycans
Journal
Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722
Informations de publication
Date de publication:
05 03 2021
05 03 2021
Historique:
received:
04
02
2021
revised:
02
03
2021
accepted:
03
03
2021
entrez:
3
4
2021
pubmed:
4
4
2021
medline:
25
8
2021
Statut:
epublish
Résumé
Glycan-protein interactions are highly specific yet transient, rendering glycans ideal recognition signals in a variety of biological processes. In human norovirus (HuNoV) infection, histo-blood group antigens (HBGAs) play an essential but poorly understood role. For murine norovirus infection (MNV), sialylated glycolipids or glycoproteins appear to be important. It has also been suggested that HuNoV capsid proteins bind to sialylated ganglioside head groups. Here, we study the binding of HBGAs and sialoglycans to HuNoV and MNV capsid proteins using NMR experiments. Surprisingly, the experiments show that none of the norovirus P-domains bind to sialoglycans. Notably, MNV P-domains do not bind to any of the glycans studied, and MNV-1 infection of cells deficient in surface sialoglycans shows no significant difference compared to cells expressing respective glycans. These findings redefine glycan recognition by noroviruses, challenging present models of infection.
Identifiants
pubmed: 33807801
pii: v13030416
doi: 10.3390/v13030416
pmc: PMC8001558
pii:
doi:
Substances chimiques
Blood Group Antigens
0
Capsid Proteins
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Références
Lancet Infect Dis. 2018 May;18(5):545-553
pubmed: 29396001
Curr Protoc Microbiol. 2014 May 01;33:15K.2.1-61
pubmed: 24789596
Glycobiology. 2010 Jun;20(6):718-23
pubmed: 20154292
EMBO J. 2003 Sep 1;22(17):4346-55
pubmed: 12941687
J Virol. 2001 Aug;75(15):6884-93
pubmed: 11435568
J Phys Chem B. 2015 Sep 3;119(35):11466-72
pubmed: 26260011
J Biomol NMR. 2008 May;41(1):5-7
pubmed: 18398685
Infect Immun. 1988 Jul;56(7):1730-7
pubmed: 3290105
Angew Chem Int Ed Engl. 2015 Oct 5;54(41):12014-9
pubmed: 26329854
Glycobiology. 2017 Jan;27(1):80-86
pubmed: 27496762
Viruses. 2010 Apr;2(4):1011-49
pubmed: 21994669
J Virol. 2012 Jan;86(1):284-92
pubmed: 22031945
Proc Natl Acad Sci U S A. 1996 Oct 15;93(21):11382-8
pubmed: 8876144
Proteins. 2005 Jun 1;59(4):687-96
pubmed: 15815974
J Virol. 2007 Jun;81(11):5949-57
pubmed: 17392366
Biochim Biophys Acta. 2013 Aug;1828(8):1840-5
pubmed: 23528203
J Am Chem Soc. 2019 Oct 16;141(41):16303-16311
pubmed: 31533424
J Virol. 2010 Jun;84(11):5695-705
pubmed: 20335262
mSphere. 2016 Mar 30;1(2):
pubmed: 27303720
Glycobiology. 2009 Mar;19(3):309-20
pubmed: 19054801
Gastroenterology. 2002 Jun;122(7):1967-77
pubmed: 12055602
PLoS Pathog. 2011 Jul;7(7):e1002152
pubmed: 21811409
J Virol. 2009 May;83(9):4092-101
pubmed: 19244326
Angew Chem Int Ed Engl. 2012 Jan 23;51(4):928-32
pubmed: 22170811
Sci Rep. 2015 Jul 06;5:11784
pubmed: 26146020
Proc Natl Acad Sci U S A. 2008 Apr 1;105(13):5219-24
pubmed: 18353982
Annu Rev Immunol. 1984;2:103-26
pubmed: 6085749
Science. 2016 Aug 26;353(6302):933-6
pubmed: 27540007
Glycobiology. 2018 Jul 1;28(7):488-498
pubmed: 29562255
J Virol. 2019 Jan 4;93(2):
pubmed: 30355683
J Am Chem Soc. 2014 Sep 10;136(36):12631-7
pubmed: 25105447
J Virol. 2012 May;86(10):5584-93
pubmed: 22438544
Prog Nucl Magn Reson Spectrosc. 2013 Aug;73:1-16
pubmed: 23962882
Glycobiology. 2009 Nov;19(11):1176-84
pubmed: 19625485
Glycobiology. 2017 Nov 1;27(11):1027-1037
pubmed: 28973640
J Gen Virol. 2019 Oct;100(10):1393-1406
pubmed: 31483239
J Virol. 2016 Aug 12;90(17):7703-14
pubmed: 27307569
Glycobiology. 2015 Feb;25(2):170-80
pubmed: 25395406
Proc Natl Acad Sci U S A. 2016 Oct 11;113(41):E6248-E6255
pubmed: 27681626
Arch Virol. 2004 Sep;149(9):1673-88
pubmed: 15593412
J Am Chem Soc. 2006 Oct 18;128(41):13529-38
pubmed: 17031966
J Infect Dis. 2003 Jul 1;188(1):19-31
pubmed: 12825167
mBio. 2020 Mar 17;11(2):
pubmed: 32184242
Biomol NMR Assign. 2020 Apr;14(1):123-130
pubmed: 31993958
Curr Opin Virol. 2018 Aug;31:33-42
pubmed: 29754860
Nat Rev Microbiol. 2016 Jul;14(7):421-33
pubmed: 27211790
Proc Natl Acad Sci U S A. 2018 Sep 25;115(39):E9201-E9210
pubmed: 30194229
Glycobiology. 2013 Mar;23(3):276-85
pubmed: 23118206
Carbohydr Res. 2000 Feb 25;324(3):161-9
pubmed: 10724530
Nat Cell Biol. 2010 Jan;12(1):11-8; sup pp 1-12
pubmed: 20023649
Curr Opin Struct Biol. 2017 Jun;44:211-218
pubmed: 28591681
Nat Commun. 2019 Mar 21;10(1):1320
pubmed: 30899001
J Virol. 2007 Nov;81(22):12238-48
pubmed: 17855551
Angew Chem Int Ed Engl. 1999 Jun 14;38(12):1784-1788
pubmed: 29711196
Chembiochem. 2020 Apr 1;21(7):1007-1021
pubmed: 31644826
PLoS Pathog. 2009 Jul;5(7):e1000504
pubmed: 19578439
Nat Biotechnol. 1997 Sep;15(9):871-5
pubmed: 9306402
ACS Chem Biol. 2017 May 19;12(5):1288-1296
pubmed: 28294600
J Gen Virol. 2007 Jan;88(Pt 1):177-186
pubmed: 17170450
F1000Res. 2017 Jan 26;6:79
pubmed: 28163914
J Virol. 2018 May 14;92(11):
pubmed: 29563286
PLoS Pathog. 2015 Feb 27;11(2):e1004626
pubmed: 25723501