Neuroanatomy of melanocortin-4 receptor pathway in the mouse brain.
Western blot
central nervous system
hypothalamus
immunofluorescence
melanocortin-4 receptor
Journal
Open life sciences
ISSN: 2391-5412
Titre abrégé: Open Life Sci
Pays: Poland
ID NLM: 101669614
Informations de publication
Date de publication:
2020
2020
Historique:
received:
14
02
2020
revised:
27
04
2020
accepted:
01
05
2020
entrez:
5
4
2021
pubmed:
6
4
2021
medline:
6
4
2021
Statut:
epublish
Résumé
Melanocortin-4 receptors (MC4Rs) are key regulators of energy homeostasis and adipose deposition in the central nervous system. Considering that MC4R expression regions and function-related research mainly focus on the paraventricular nucleus (PVN), little is known about their distribution throughout the mouse brain, although its messenger RNA distribution has been analyzed in the rat. Therefore, MC4R protein localization in mouse neurons was the focus of this study. MC4R protein distribution was assessed in mice through immunofluorescence and Western blotting. MC4R was differentially expressed throughout the arcuate nucleus (ARC), nucleus of the solitary tract (NTS), raphe pallidus (RPa), medial cerebellar nucleus, intermediolateral nucleus, and brainstem. The highest MC4R protein levels were found in the ARC and ventromedial hypothalamic nucleus, while they were significantly lower in the parabrachial nucleus and NTS. The lowest MC4R protein levels were found in the PVN; there was no difference in the protein levels between the area postrema and RPa. These data provide a basic characterization of MC4R-expressing neurons and protein distribution in the mouse brain and may aid further research on its role in energy homeostasis.
Identifiants
pubmed: 33817246
doi: 10.1515/biol-2020-0063
pii: biol-2020-0063
pmc: PMC7874588
doi:
Types de publication
Journal Article
Langues
eng
Pagination
580-587Informations de copyright
© 2020 Kun Wang et al., published by De Gruyter.
Déclaration de conflit d'intérêts
Conflict of interest: The authors state no conflict of interest.
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