Apocynin prevents cigarette smoking-induced loss of skeletal muscle mass and function in mice by preserving proteostatic signalling.


Journal

British journal of pharmacology
ISSN: 1476-5381
Titre abrégé: Br J Pharmacol
Pays: England
ID NLM: 7502536

Informations de publication

Date de publication:
08 2021
Historique:
revised: 08 03 2021
received: 16 10 2020
accepted: 24 03 2021
pubmed: 6 4 2021
medline: 29 7 2021
entrez: 5 4 2021
Statut: ppublish

Résumé

Skeletal muscle dysfunction is a major comorbidity of chronic obstructive pulmonary disease (COPD). This type of muscle dysfunction may be a direct consequence of oxidative insults evoked by cigarette smoke (CS) exposure. The present study examined the effects of a potent Nox inhibitor and reactive oxygen species (ROS) scavenger, apocynin, on CS-induced muscle dysfunction. Male BALB/c mice were exposed to either room air (sham) or CS generated from nine cigarettes per day, 5 days a week for 8 weeks, with or without the coadministration of apocynin (5 mg·kg Eight weeks of CS exposure caused muscle dysfunction in mice, reflected by 10% loss of muscle mass and 54% loss of strength of tibialis anterior which were prevented by apocynin administration. In C2C12 myotubes, direct exposure to H Targeted pharmacological inhibition of Nox-derived ROS may alleviate the lung and systemic manifestations in smokers with COPD.

Sections du résumé

BACKGROUND AND PURPOSE
Skeletal muscle dysfunction is a major comorbidity of chronic obstructive pulmonary disease (COPD). This type of muscle dysfunction may be a direct consequence of oxidative insults evoked by cigarette smoke (CS) exposure. The present study examined the effects of a potent Nox inhibitor and reactive oxygen species (ROS) scavenger, apocynin, on CS-induced muscle dysfunction.
EXPERIMENTAL APPROACH
Male BALB/c mice were exposed to either room air (sham) or CS generated from nine cigarettes per day, 5 days a week for 8 weeks, with or without the coadministration of apocynin (5 mg·kg
KEY RESULTS
Eight weeks of CS exposure caused muscle dysfunction in mice, reflected by 10% loss of muscle mass and 54% loss of strength of tibialis anterior which were prevented by apocynin administration. In C2C12 myotubes, direct exposure to H
CONCLUSION AND IMPLICATIONS
Targeted pharmacological inhibition of Nox-derived ROS may alleviate the lung and systemic manifestations in smokers with COPD.

Identifiants

pubmed: 33817783
doi: 10.1111/bph.15482
pmc: PMC8362135
doi:

Substances chimiques

Acetophenones 0
Smoke 0
acetovanillone B6J7B9UDTR

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

3049-3066

Subventions

Organisme : National Health and Medical Research Council
ID : APP1138915
Organisme : National Health and Medical Research Council
ID : APP1084627

Informations de copyright

© 2021 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society.

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Auteurs

Stanley M H Chan (SMH)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Ivan Bernardo (I)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Chanelle Mastronardo (C)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Kevin Mou (K)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Simone N De Luca (SN)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Huei Jiunn Seow (HJ)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Aleksandar Dobric (A)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Kurt Brassington (K)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Stavros Selemidis (S)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Steven Bozinovski (S)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

Ross Vlahos (R)

School of Health and Biomedical Sciences, RMIT University, Melbourne, Victoria, Australia.

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Classifications MeSH