Multicentre study of the burden of multidrug-resistant bacteria in the aetiology of infected diabetic foot ulcers.
anaerobic culture
antibiotic
bacteria
diabetic foot
infection
multidrug-resistance
samples
ulcers
Journal
African journal of laboratory medicine
ISSN: 2225-2002
Titre abrégé: Afr J Lab Med
Pays: South Africa
ID NLM: 101603205
Informations de publication
Date de publication:
2021
2021
Historique:
received:
03
05
2020
accepted:
22
10
2020
entrez:
7
4
2021
pubmed:
8
4
2021
medline:
8
4
2021
Statut:
epublish
Résumé
Infected diabetic foot ulcer (IDFU) is a public health issue and the leading cause of non-traumatic limb amputation. Very few published data on IDFU exist in most West African countries. The study investigated the aetiology and antibacterial drug resistance burden of IDFU in tertiary hospitals in Osun state, Nigeria, between July 2016 and April 2017. Isolates were cultured from tissue biopsies or aspirates collected from patients with IDFU. Bacterial identification, antibiotic susceptibility testing and phenotypic detection of extended-spectrum beta-lactamase and carbapenemase production were done by established protocols. Specific resistance genes were detected by polymerase chain reaction. There were 218 microorganisms isolated from 93 IDFUs, comprising 129 (59.2%) Gram-negative bacilli (GNB), 59 (27.1%) Gram-positive cocci and 29 (13.3%) anaerobic bacteria. The top five facultative anaerobic bacteria isolated were: Multidrug-resistant facultative anaerobic bacteria are overrepresented as agents of IDFU. A relatively low proportion of the aetiological agents were anaerobic bacteria.
Sections du résumé
BACKGROUND
BACKGROUND
Infected diabetic foot ulcer (IDFU) is a public health issue and the leading cause of non-traumatic limb amputation. Very few published data on IDFU exist in most West African countries.
OBJECTIVE
OBJECTIVE
The study investigated the aetiology and antibacterial drug resistance burden of IDFU in tertiary hospitals in Osun state, Nigeria, between July 2016 and April 2017.
METHODS
METHODS
Isolates were cultured from tissue biopsies or aspirates collected from patients with IDFU. Bacterial identification, antibiotic susceptibility testing and phenotypic detection of extended-spectrum beta-lactamase and carbapenemase production were done by established protocols. Specific resistance genes were detected by polymerase chain reaction.
RESULTS
RESULTS
There were 218 microorganisms isolated from 93 IDFUs, comprising 129 (59.2%) Gram-negative bacilli (GNB), 59 (27.1%) Gram-positive cocci and 29 (13.3%) anaerobic bacteria. The top five facultative anaerobic bacteria isolated were:
CONCLUSION
CONCLUSIONS
Multidrug-resistant facultative anaerobic bacteria are overrepresented as agents of IDFU. A relatively low proportion of the aetiological agents were anaerobic bacteria.
Identifiants
pubmed: 33824857
doi: 10.4102/ajlm.v10i1.1261
pii: AJLM-10-1261
pmc: PMC8008032
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1261Informations de copyright
© 2021. The Authors.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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